(Beyond Pesticides, November 5, 2024) Published in the journal Frontiers in Toxicology, a recent study uncovers serious flaws in the pesticide registration process at the U.S. Environmental Protection Agency (EPA) with an in-depth evaluation of the agency’s failure to protect the public from the harmful effects of five neonicotinoid (neonic) insecticides—as mandated by the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) and amendments, including Food Quality Protection Act (FQPA) of 1996. This coincides with EPA’s ongoing review to renew their approval for the next 15 years (set to be announced in 2025). The report is based on the first comprehensive assessment of unpublished rodent-based Developmental Neurotoxicity (DNT) studies, conducted between 2000-2003 and submitted by pesticide manufacturers as part of the registration process. All five neonicotinoids evaluated—acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam—are associated with significant shrinkage of brain tissue at the highest dosage, according to EPA data reports (see acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam). However, with little or no data regarding the chemicals’ impacts at low and mid-level dosages, EPA has either failed to find a “No Observed Adverse Effect Level” (NOAEL) or, seemingly at random, set the NOAEL at the mid-level dosage. The evaluation suggests that perinatal exposure to […]

(Beyond Pesticides, September 18, 2024) A literature review in Trends in Analytical Chemistry analyzes scientific articles from the last ten years from around the globe that identify more than 300 pesticides in bee pollen. Bee pollen, often used as an edible dietary supplement, is not regulated for pesticide residues, which sparks concern for human exposure due to contamination with pesticides, heavy metals, metalloids, and mycotoxins. “Bee pollen is a food supplement that is receiving increasing attention for its nutraceutical and therapeutic properties. However, several uncertainties on the safety of this beekeeping product still exist. The present work addressed this issue through the critical evaluation of 61 studies, published over the 2014–2024 period,” the Spanish authors state. Bee pollen is produced by honey bees. After they forage on flowers and gather pollen on their hind legs (in pollen baskets or corbiculae) to transport back to the hive, it is moistened with nectar and salivary secretions to create bee pollen in the form of pellets. While the composition of bee pollen can vary between geographical locations with different flowers, the studies reviewed all utilize mass spectrometry to pinpoint pesticides, as well as mycotoxins (created by naturally occurring mold spores), that threaten human […]

(Beyond Pesticides, June 21, 2024) A recent review of the scientific literature, published in Science of The Total Environment, analyzes multiple species of bees on a molecular level to better understand the poisoning mechanisms that could, as the authors see it, inform chemical risk assessments with more precision. The mechanisms “implicated in the tolerance of bees to specific pesticides, and thus as determinants of insecticide sensitivity, … include metabolic detoxification, insecticide target proteins, the insect cuticle and bee gut microbiota,” the authors write. This review references more than 90 studies performed over the last 30+ years, with most being published in the last 5-10 years, as the understanding and importance of molecular determinants of bee sensitivity has emerged. Pollinators, such as bees, provide crucial ecosystem services by pollinating both wild plants and essential crops. The exposure these insects are subjected to threatens their existence, which occurs through pesticide contamination that can lead to impacts on growth and development or even colony collapse.    “While bees have only been exposed to human-made pesticides over the recent past (last 80 years) they have co-evolved with plants and fungi which produce a range of xenobiotics, including plant allelochemicals and mycotoxins,” the authors state. […]

(Beyond Pesticides, June 12, 2024) A study of pesticide contaminated algae finds that the disruption of algal communities has a devastating effect on the health of the aquatic food web. The study findings show that contact with pesticides can result in changes to “algal physiology, causing tissue injury, developmental delay, genotoxicity, procreative disruption, and tissue biomagnification” that alters the dominance of algae species in the environment. This in turn “can impact higher trophic levels and have a domino effect on the aquatic food web. It is possible for biodiversity to disappear, reducing ecosystem stability and resistance to environmental alterations,” the authors state. The study, a worldwide literature review conducted by researchers from India, South Korea, and Saudi Arabia, appears in Aquatic Toxicology.  The health of aquatic ecosystems is at risk with indirect effects on nontarget species from pesticides in the environment. This includes impacts on species of fish, invertebrates, microbial communities, and marine mammals. In explaining the importance of extensively studying effects of pesticides, the researchers note, “Different pesticide classes have different chemical structures, which define their modes of action and affect how they interact with both target and nontarget organisms.” Because of this, the range of effects seen from […]

(Beyond Pesticides, May 16, 2024) In a recent study at the Institute of Biochemistry and Molecular Biology at Ulm University in Germany, published in Current Research in Toxicology, scientists exposed embryos of South African clawed frogs (Xenopus laevis) to three neonicotinoids (NEOs), which led to developmental effects down to a molecular level. These frogs are a well-established model species often used in ecotoxicology studies as bioindicators for overall environmental and ecosystem health. When amphibian species like Xenopus laevis are exposed to contaminants in the water, it leads to negative impacts in the food chain and harms biodiversity. The study concludes that exposure to NEOs directly or through contaminated water leaves entire ecosystems vulnerable.    The NEOs that the embryos were subjected to include imidacloprid (IMD), thiamethoxam (TMX), and its metabolite clothianidin (CLO). NEOs are a class of insecticides that target the central nervous system of insects and lead to death. These insecticides pose a potential hazard to nontarget organisms, such as animals and humans, since they are persistent in the environment and “are found in natural waters as well as in tap water and human urine in regions where NEOs are widely used,” this study states. The authors continue by […]

(Beyond Pesticides, April 11, 2024) Scientists are moving forward in testing an agroecological method of “push-pull” pest management (reducing the attractiveness of the target organism and luring pest insects towards a trap) to fight the Asian citrus psyllid (ACP) in Florida orange groves, as it spreads a plant disease known as the pathogenic bacteria huanglongbing (HLB), also known as citrus greening, which is deadly to citrus trees. The disease is spread by the pathogenic bacteria Candidatus Liberibacter asiaticus (CLas).  The chemical-intensive, or conventional, citrus industry is under intense pressure to find alternatives, as synthetic antibiotic use for this purpose has been successfully challenged in court. ACP is the carrier, or vector, for HLB, spreading it through the citrus groves and killing the trees. The chemical-intensive industry has focused on using antibiotics, which the environmental and public health community has rejected because of serious medical concerns associated with life-threatening bacterial resistance to antibiotics used to protect humans. A federal district court decision in December 2023 found illegal the U.S. Environmental Protection Agency’s (EPA) decision to register the antibiotic streptomycin in Florida citrus without adequate review of its impact on endangered species. The streptomycin lawsuit, filed in 2021 by a coalition of […]

(Beyond Pesticides, March 15, 2024) Toxic pesticides harm all beings and ecosystems, including coral reefs. Large benthic foraminifera (LBF) are single-celled organisms found on reefs that face adverse metabolic impacts after exposure to the weed killer glyphosate and insecticide imidacloprid, according to a study published in Marine Pollution Bulletin. The study found that “even the lowest doses of the fungicide and herbicide caused irreparable damage to the foraminifera and their symbionts.” Beyond Pesticides reiterates our mission of banning toxic petrochemical pesticides by 2032 and that this goal applies to land and water exposure to pesticides. LBFs are typically used as bioindicators for coral health because they are found in substantial quantities and gathering data is not intrusive or damaging to reef health. Researchers in this study screened three different herbicides (one insecticide, one fungicide, and one herbicide) at three different concentration levels. The experiments were performed in six well-samples, each with 10mL of filtered artificial seawater and a singular LBF. The control plates are artificial seawater and the experimental plates include artificial seawater with the addition of three pesticides (imidacloprid, glyphosate, and tebuconazole). Each pesticide was applied at low, medium, and high doses to measure the direct impacts of each […]

(Beyond Pesticides, March 11, 2024) Inside a recent disagreement between the Office of the Inspector (OIG) and the U.S. Environmental Protection Agency’s (EPA) Office of Pesticide Programs (OPP) on the agency’s review of pet flea and tick collars—leading to thousands of deaths and poisonings—is a basic question of the adequacy of pesticide regulation. The disagreement is over the cause of 105,354 incident reports, including 3,000 pet deaths and nearly 900 reports of human injury, and the February 2025 OIG report’s conclusion that “[EPA] has not provided assurance that they can be used without posing unreasonable adverse effects to the environment, including pets.” While the disagreement focuses on a number of EPA process failures, Beyond Pesticides urges that the findings advance the need for the agency to address a key element of chemical mixtures in pesticide products not currently evaluated, potential synergistic effects—the increased toxic potency created by pesticide and chemical combinations not captured by assessing product ingredients individually.  Key to the dispute is what many see as a foundational failure of EPA to evaluate the effect of pesticide mixtures and full formulations of pesticide end products, a longstanding criticism of the agency’s pesticide registration process, which focuses on pesticide products’ active […]

(Beyond Pesticides, March 6, 2024) With over 2,500 pet deaths and 900 reports of adverse effects to people, an Office of Inspector General (OIG) report, published on February 29, 2024, reveals multiple systemic failures by the U.S. Environmental Protection Agency’s (EPA) Office of Pesticide Programs (OPP), citing inadequate safety reviews of Seresto pet collars. The report, The EPA Needs to Determine Whether Seresto Pet Collars Pose an Unreasonable Risk to Pet Health, concludes, “The EPA’s response to reported pesticide incidents involving Seresto pet collars has not provided assurance that they can be used without posing unreasonable adverse effects to the environment, including pets.” At the time the animal effects made headlines in 2021, the agency defended the product’s registration, telling the media that, despite these incidents, EPA deemed Seresto collars “‘eligible for continued registration’ based on best available science, including incident data… No pesticide is completely without harm, but EPA ensures that there are measures on the product label that reduce risk.” Despite the scathing criticism, EPA maintains the position that it conducted an adequate review of the two active insecticide ingredients in the pet collars—the neurotoxic insecticide flumethrin, and the notorious neonicotinoid imidacloprid—proven to have adverse effects on the endocrine system as […]

(Beyond Pesticides, January 25, 2024) Legislative efforts to curtail some life-threatening pesticides associated with birds and bees (and other pollinators) decline were weakened in New York State at the end of December 2023 as the governor negotiated and stripped elements of a bill relating to agriculture that had passed the legislature—again illustrating the grip of the agrichemical industry on public policy intended to begin to address the crisis in ecosystem collapse. (See “Study Cites Insect Extinction and Ecological Collapse.”) In passing the Birds and Bees Protection Act, New York joined New Jersey, Nevada, and Maine in banning most nonagricultural uses of neonicotinoid (neonic) insecticides, but, in last-minute changes to avoid the governor’s veto, failed to phase out corn, soybean, and wheat seeds coated with these chemicals. [Pointing to an exemption in federal law that has been challenged by advocates, the U.S. Environmental Protection Agency (EPA) does not regulate treated or coated seeds as pesticides despite their toxic pesticidal properties.] In New York State, the governor can, in consultation with the leadership of the legislative branch, negotiate language changes (called Chapter Amendments) in legislature-passed legislation (originally enacted) before deciding to sign it into law or can simply choose to veto the legislation. […]

(Beyond Pesticides, December 8, 2023) Nine pesticides have been found in nearly 40% of nonorganic conventional baby foods tested, according to a study conducted by Environmental Working Group (EWG). The study found no residues of the pesticides studied in a sample of certified organic baby food. While the study finds no traces of the highly neurotoxic insecticide chlorpyrifos, associated with brain damage in children, the chemical has been allowed back on the agricultural market after being removed in 2021—raising an alarm for parents who purchase baby food with ingredients grown in chemical-intensive (“conventional”) agriculture. In November 2023, a three-judge panel of the Eighth Circuit Court of Appeals reversed a 2021 U.S. Environmental Protection Agency (EPA) decision to ban chlorpyrifos’ agricultural uses, which came after a 2021 Ninth Circuit Court of Appeals decision found that the agency’s inaction violated federal pesticide law. Because of its neurotoxic effects on children, EPA had in 2000 negotiated Dow Chemical’s voluntary cancellation of most residential uses of the chemical, but left virtually all of the chemical’s agricultural uses in place. While the EWG study focuses on pesticide residues in food and the hazards associated with ingestion of dangerous chemicals, raising alarms, purchasing baby food processed with nonorganic […]

(Beyond Pesticides, October 17, 2023) In the past month, Paris, France has witnessed a surge in bed bug populations. From public transportation to hotels, hostels, and movie theatres, bed bugs are posing a threat to the city’s two million residents and potentially a broader global population as the infestation spreads.   This resurgence of bed bugs in Paris is not unique. For centuries, these pests have been both adaptable and persistent, presenting an enduring challenge to pest control. However, the current surge in bed bug infestations is not merely a revival of a longstanding problem; it is a complex issue intertwined with the development of resistance to insecticides, mainly through a mechanism known as knockdown resistance. This mechanism, along with three other main resistance mechanisms, has enabled these insects to defy chemical-intensive control methods  Knockdown resistance is a significant factor contributing to the resistance exhibited by bed bugs to insecticides, especially pyrethroids. The mechanism plays a central role in countering the action of these insecticides, which target the nervous system of bed bugs, causing paralysis and eventual death. Knockdown resistance provides the genetic adaptation that provides bed bug populations with resistance to insecticides. It inhibits the effectiveness of certain insecticides. Bed […]

(Beyond Pesticides, September 19, 2023) Farmworkers in Paarl, South Africa took to the streets on Friday, September 8, demanding an end to the indiscriminate importation and use of pesticides, herbicides, and fungicides containing substances prohibited by the European Union (EU). This protest is part of a broader global trend of outcry against systemic issues of environmental racism that disproportionately burden communities with environmental and health risks.   Organized by the Women on Farms Project, the protesters marched to the headquarters of Bayer. The German pharmaceutical, biotechnology, and pesticide company, responsible for producing and exporting agrochemicals known to be toxic to ecosystem and human health, has previously faced multiple lawsuits, including a multimillion-dollar one linking their glyphosate weed killer products (Roundup®) to non-Hodgkin Lymphoma. At the Bayer office, the protesters presented a memorandum demanding an end to the importation and use of EU-prohibited substances.    Protesters sought to expose the hypocritical tactics European agrochemical companies use to sell products in developing nations, even when those products are deemed unsafe in their home countries. Numerous farmworkers, like victim-turned-activist Antie Dina, spoke out about their health issues from petrochemical exposure. In her talk, Dina emphasizes that, “… enough is enough, we do not want any […]

(Beyond Pesticides, February 14, 2023) The waterways that flow into the Great Lakes are experiencing year-round pesticide contamination that exceeds benchmarks meant to protect aquatic life, according to research published in Environmental Toxicology and Chemistry by scientists at the U.S. Geological Survey (USGS). “What you use makes it into the water,” study coauthor Sam Oliver, PhD, told the Milwaukee Journal Sentinel. These data buttress growing calls from pesticide reform advocates that new laws are needed to protect the nation’s increasingly threatened waters. USGS scientists conducted their analysis on 16 tributaries that feed into the Great Lakes, including sites that correspond to urban, agricultural, and undeveloped land. Samples were taken at locations closest to the lake the tributary flowed into over a period of roughly one year from October 2015 to September 2016. Each sample was tested for 231 pesticides and their breakdown products. Researchers used aquatic life benchmarks set by the U.S. Environmental Protection Agency (EPA), and created a relative hazard index (RHI) for the study to evaluate whether specific sites should be prioritized for further protections.   Across every sampled tributary, pesticides were found. Accordingly, 96% (190 out of 198) of samples taken contained pesticides or their breakdown products. […]

(Beyond Pesticides, January 11, 2022) Neonicotinoid insecticides can have detrimental effects on liver health, according to research published in the Journal of Hazardous Materials. While this is the first study to investigate how these chemicals harm the liver, there is increasing evidence that neonicotinoids, otherwise notorious for their effects on pollinators and aquatic life, can cause direct harm to human health. As the U.S. Environmental Protection Agency (EPA) continues to protect the pesticide industry from any measure of meaningful regulation around these hazardous products, the job falls to advocates to place pressure on elected officials to make the changes necessary to safeguard long-term health and well-being. Scientists postulated that neonicotinoids are neither metabolized by the liver nor excreted by urine. To test that hypothesis, 201 individuals from a hospital in China were enrolled into a study. Of the enrolled,  81 were cancer patients, and 120 were not. These individuals underwent a procedure called endoscopic retrograde cholangiopancreatography whereby samples of their bile, a fluid produced in the liver, were retrieved and analyzed. Researchers also performed a series of blood tests, measuring a range of biomarkers, including cholesterol, bilirubin, bile acids, white blood cells, platelets, and others. Lastly, scientists determined the amount […]

(Beyond Pesticides, November 15, 2022) Spraying a flowering plant with synthetic fertilizers makes it less attractive to bumblebees, according to research published this month in PNAS Nexus.  “A big issue is thus—agrochemical application can distort floral cues and modify behaviour in pollinators like bees,” said study author Ellard Hunting, PhD, of the University of Bristol, UK. The findings underscore the limited understanding that proponents of chemical agriculture have for the complex processes that food production relies upon and reinforce calls for a broad scale transition to regenerative, organic farming practices. Scientists began with the knowledge that spray applications of various agrichemicals affect the visitation patterns of bumblebees and other pollinators through a range of different processes. Past research finds that notorious bee-killing neonicotinoid insecticides not only kill bees outright, but also result in a range of complex damage, including their ability to impede bees’ olfactory senses and adversely affect their vision and flying ability. Other chemicals like glyphosate weaken bees’ ability to distinguish between colors.   A growing area of research is investigating the ways in which pollinators use static electric fields surrounding flowers to find food sources. A 2013 study found that bumblebees use floral electrical fields to discriminate […]

(Beyond Pesticides, October 6, 2022) A new study finds alarmingly high levels of PFAS (Per- and Polyfluoroalkyl Substances) “forever chemicals” in commonly used pesticides, calling into question assurances from the U.S. Environmental Protection Agency (EPA) that contamination is limited to storage containers. For some pesticides, PFAS levels are nearly one billion times higher than the EPA’s recently updated Health Advisory for the PFAS chemical PFOS. “If the intent was to spread PFAS contamination across the globe there would be few more effective methods than lacing pesticides with PFAS,” said Kyla Bennett, PhD, of the nonprofit Public Employees for Environmental Responsibility. “These findings point to an appalling regulatory breakdown by EPA.” A team of researchers based in Texas, including scientists from Texas Tech and the U.S. Department of Agriculture’s (USDA) Cropping Systems Research Laboratory, participated in the study published in the Journal of Hazardous Materials Letters. Ten different formulated pesticide products were tested for 24 different PFAS substances. The pesticide products selected were determined based on unexpected PFAS contamination at USDA’s research facility. During the course of conducting a separate study on plant uptake of PFAS, detectable levels of PFAS were found in plants intended to be used as unexposed controls. […]

(Beyond Pesticides, September 27, 2022) Exposure to widely used neonicotinoid insecticides harms amphibians at multiple life stages, adversely affecting their ability to survive in the wild, according to research published in the Journal of Zoology. As long-lived, systemic insecticides, neonicotinoids are consistently found in U.S. waterways,  often above federal safety limits, making these findings particularly dangerous for frogs and other amphibians throughout the country. As troubling data piles up on this class of dangerous insecticides, which are damaging pollinators, birds, deer, aquatic wildlife, and human health, it is left to the public to place pressure on federal regulators and members of Congress to act. To understand the impact of neonicotinoids on amphibian life stages, researchers conducted a range of  experiments. These were designed to investigate how exposure to the neonicotinoid imidacloprid affected larval survival, sexual development, locomotor skills, and avoidance behavior of the wood frog (Rana sylvatica). Larval survival was examined by exposing tadpoles to 10 parts per billion (ppb) of imidacloprid, a rate lower than the lethal concentration expected to kill half of other frogs species in acute toxicity tests. Four treatment protocols were established, adding the variable of natural pond drying to half of the tested frogs to […]

(Beyond Pesticides, August 16, 2022) Over half of all food samples tested by the U.S. Food and Drug Administration (FDA) contain the residues of at least one pesticide, and one in ten samples have levels that violate legal limits established by the U.S. Environmental Protection Agency (EPA). These findings, published by FDA this month in its 2020 Pesticide Residue Monitoring Report, are simply par for the course for government regulators, as FDA indicates the 2020 results “were consistent with recent years.” However, while reporting on the dangerous pesticides present in U.S. food has become routine for FDA, more and more Americans are rejecting regular exposure to unnecessary toxics in their food by going organic with their food choices, planting their own pesticide-free gardens, and encouraging their elected officials to embrace safer, sustainable land care policies.   FDA has conducted a review of pesticide residues on food on an annual basis since 1987, evaluating both domestic and imported foodstuffs into the US market. While EPA sets “pesticide tolerances,” also known as “maximum residue levels,” of allowed pesticide residues on certain foods, FDA (and USDA, for some specific items like meat, poultry, and eggs) is tasked with enforcing these provisions. Pesticide tolerances, […]

(Beyond Pesticides, April 1, 2022) According to reporting by E&E’s Greenwire, internal emails at the U.S. Environmental Protection Agency (EPA) show that career scientists at the agency expressed worry about pesticide-laced pet collars, such as the notorious Seresto flea and tick collars, but that EPA managers “instructed them to avoid documenting those worries in publicly accessible records.” The emails were released pursuant to a 2021 FOIA (Freedom of Information Act) lawsuit, brought by the Center for Biological Diversity (CBD), that sought records of internal communications. The documents evidence staff concern about the collars that has not been a part of EPA’s public communications on the subject. EPA staff, in the emails, expressed a range of degrees of outrage at managers’ behavior and at the very registration of the product, given the significant harms. Seresto collars are plastic pet collars embedded with pesticides designed to kill fleas, ticks, and lice; they contain the active ingredients flumethrin and imidacloprid. Flumethrin, a chemical in the pyrethroid class of synthetic neurotoxic insecticides, has been linked repeatedly to neurological issues, such as seizures and learning disabilities in children, to gastrointestinal distress, and to damage to nontarget invertebrates, according to EPA’s own analysis. Imidacloprid is a commonly used pesticide linked to […]

(Beyond Pesticides, January 4, 2021) Chronic exposure to pesticides used in conventional forestry operations runoff and harm soft shell clams, according to a recent study published in Science of the Total Environment, entitled “The silence of the clams: Forestry registered pesticides as multiple stressors on soft-shell clams.” Rather than focusing on the impact of a single chemical, researchers analyzed the combined effects of several pesticides. “This is an important data gap to fill as research on these compounds’ toxicity typically focuses on individual compound effects at high concentrations to determine lethality, which while necessary for understanding compound toxicity, can miss sublethal effects that can have long term impacts on these systems,” said lead author Allie Tissot of Portland State University. The soft shell clam, Mya arenaria, is found to be widespread in coastal areas in both the western and eastern U.S., and is often eaten in stews or chowders. A recent study found a range of chemical contaminants detected in Oregon populations of these species, prompting researchers to further investigate the impact of these exposures. An experiment was set up with tanks to mimic a seabed, and eight different groups of 11 clams were established and treated with various amount […]

(Beyond Pesticides, December 7, 2021) One exposure. That’s all it takes for wild bees to experience declines in reproduction and population growth from neonicotinoid insecticides, according to research recently published in the Proceedings of the National Academy of Sciences (PNAS). This incredible sensitivity is exactly the sort of process that could rapidly drive pollinator species into extinction. It is the sort of finding that one would expect government agencies tasked with protecting the environment to discern. Yet, regulators at the U.S. Environmental Protection Agency’s (EPA) Office of Pesticide Programs have consistently failed to listen and meaningfully respond to the latest science. As this is done, the agency is fully aware that ever more pollinators are slated for endangered status, jeopardizing our agricultural economy, ecosystem stability, and the joy we all gain from watching our favorite pollinators flit about the landscape. Over the course of two years, researchers established a crossed experiment with ground-nesting blue orchard bees (Osmia lignaria). These pollinators, native to North America, overwinter and nest in narrow holes or tubes, making them particularly sensitive to ground-based pesticide applications. Researchers conducted their study during the first year by exposing a group of larval bees to the neonicotinoid imidacloprid through […]

(Beyond Pesticides, September 15, 2021) Toxic pesticide use, and glyphosate in particular, degrades the health of freshwater ecosystems by harming species that form the basis of aquatic food chains, according to research published by scientists at McGill University. In a series of studies, scientists investigated how freshwater bacteria and zooplankton were affected by varying levels the weed killer glyphosate, the neonicotinoid insecticide imidacloprid, and nutrient levels. “Because plankton form the foundation of the food chain in freshwater ecosystems, it is very important to understand how plankton communities respond to widely used pesticides,” said Jesse Shapiro, PhD, an Associate Professor in McGill’s Department of Microbiology and Immunology. “Our research shows that the structure of these communities can be impaired under currently acceptable North American water quality guidelines.” Two separate experiments were conducted under similar conditions in order to properly investigate the effects of pesticide exposure on either zooplankton or freshwater bacteria. For both studies, target species were exposed to varying rates of glyphosate, imidacloprid, or both chemicals at either high or low water nutrient levels. Researchers conducted this study by establishing a series of outdoor experimental ponds, intended to mimic freshwater ecosystems by using Lake water and evenly distributing organisms throughout […]