(Beyond Pesticides, March 11, 2025) A study in Toxics analyzes ipconazole, a triazole fungicide often used as a coating on treated seeds and as a foliar treatment on the leaves of plants. “Triazole pesticides are widely used throughout the world, but their abuse causes toxic effects in non-targeted organisms,” the researchers state. In the current study, unintended reproductive effects are noted in male sheep (ram) and pigs (also known as porcine or swine). This research focuses on the impact of ipconazole exposure on spermatozoa (sperm) in two mammal species and finds spermiotoxicity through significantly reduced sperm viability, as well as alterations in enzyme and gene expression related to fertility.  “To our knowledge, this is the first study to evaluate the cytotoxic effect of the triazole ipconazole on mammalian spermatozoa,” the authors share. This analysis utilizes semen samples from the Reproductive Biotechnology Laboratory of the Major National University of San Marcos in Lima, Peru, the university where six of the nine researchers are Faculty of Veterinary Medicine.   “The sperm were exposed to ipconazole concentrations of 1, 5, 10, 50 and 100 µM, and to a control without ipconazole,” they say. Similar concentrations have been used in previous cytotoxicity studies with ipconazole. […]

(Beyond Pesticides, February 7, 2025) With the strong associations established in epidemiologic studies and a dearth of evidence on the actual mechanisms by which pesticides inflict their damage on the brain, a new meta-analysis identifies studies that pesticides can overwhelm cells’ defenses against them, interfere with cell communication in the brain, and disrupt the epigenetic (gene function) regulation of gene expression. In the journal Nucleus, Bilal Ahmad Mir, PhD and colleagues at the University of Kashmir in Srinagar, India, review what is known at the molecular level about pesticides’ role in brain cancer. Brain cancer is a heavy burden for its victims and their families, and attributing a cause is, as with many diseases, fraught with difficulty. But there are strong clues: For example, farming is a known risk factor. In a 2021 meta-analysis of epidemiologic studies between 1998 and 2019, analyzed here by Beyond Pesticides, farming was associated with a 13% increase in the risk of brain cancer. For farmers whose specific exposure to pesticides was documented in the studies, the increase jumped to 20 percent. So there is some reason to point a finger at synthetic chemicals. But agricultural workers are not the only ones at risk. According […]

(Beyond Pesticides, January 16, 2025) In Ecotoxicology and Environmental Safety, researchers highlight a multitude of studies on endocrine disrupting chemicals (EDCs) and endocrine disrupting pesticides (EDPs) showing epigenetic effects from exposure. These EDCs imitate the action of endocrine hormones and lead to gene damage and multigenerational adverse effects to health. “These chemicals can interfere with the normal functioning of target tissues by altering their response to hormonal signals, thereby affecting various physiological processes including reproduction, development, the nervous system, the immune system, and even the process of carcinogenesis [causing cancer],” according to the authors from Hebei Agricultural University and Chinese Academy of Agricultural Sciences.   In causing epigenetic modifications, the authors describe that EDCs can create changes “at the nuclear and mitochondrial DNA (nDNA and mtDNA) or RNA levels, without changing the underlying DNA sequence. These alterations modify the structure or conformation of DNA, influencing gene expression and, consequently, cellular function.” They continue, “The mechanisms of epigenetics include changes in DNA methylation, chromatin modifications and the involvement of certain noncoding RNAs.” In reviewing over 80 studies on EDCs, predominantly fluorinated compounds such as per- and polyfluoroalkyl substances (PFAS), the researchers provide a summary of linkages between pesticide exposure and the […]

(Beyond Pesticides, December 10, 2024) Scientists from the Engineering Research Center of Protection and Utilization of Plant Resources at Shenyang Agricultural University in China reveal adverse effects of imidacloprid on soil communities in a study published in Pesticide Biochemistry and Physiology. The researchers highlight risks to nematodes from imidacloprid exposure in maize soil, as well as potential resistance mechanisms that impact not only nematode populations but also overall soil health. Maize, or corn, a productive crop grown worldwide, is a source of food and biofuel. In assessing the soil and species in maize fields after exposure to imidacloprid at various concentrations, the study researchers assess the impact of neonicotinoid insecticides on nontarget organisms and the health of soil communities. The assessment includes an evaluation of nematodes’ survival, growth, reproduction, and chemotaxis/locomotion behavior. With a statistical analysis of lipid and lipofuscin accumulation, acetylcholinesterase (an enzyme necessary for neurotransmission) activity, and gene expression levels, the study results show that imidacloprid induces: significantly reduced abundance and diversity of nematode species. negative effects on body length, reproduction, locomotion, lipid accumulation, lipofuscin accumulation, and acetylcholinesterase activity in Caenorhabditis elegans ( elegans). the upregulation of gpa-1, cyp-35a2, fat-2, fat-6, hsp-16.41, and hsp-16.2, along with the downregulation […]

(Beyond Pesticides, November 13, 2024) A study in Chemosphere, conducted by researchers from the Institute of Biochemistry and Molecular Biology in Germany, reveals the varied lethal and sublethal effects of different glyphosate mixtures through tests on the South African clawed frog, Xenopus laevis (X. laevis). After exposing embryos to four glyphosate formulations, mortality, morphological defects, altered heartbeat rate, and impaired heart-specific gene expression are observed. Glyphosate, an herbicide and popular weed killer in many Roundup® products, is one of the most commonly detected pesticides in waterbodies worldwide, threatening aquatic organisms and overall biodiversity. This study investigates the effects of Glyphosat TF, Durano TF, Helosate 450 TF, and Kyleo, four formulations containing glyphosate, as compared to the effects of pure glyphosate on embryonic development in amphibians. The formulations consist of varying concentrations of the active ingredient glyphosate, as well as other active and inert ingredients. The authors share that, “Glyphosat TF contains 34% glyphosate and 10–20% d-glucopyranose, while Durano contains 39–44% glyphosate and 1–5% N–N-dimethyl-C12-C14-(even numbered)-alkyl-1-amines. In Helosate most of the ingredients are listed – 50–70% glyphosate, 1–10% isopropylamine, 1–3% lauryl dimethyl betaine, 0.25–1% dodecyl dimethylamine. Kyleo only lists the active ingredients glyphosate (27.9%) and 2,4-D (32%).” 2-cell stage embryos (early […]

(Beyond Pesticides, November 5, 2024) Published in the journal Frontiers in Toxicology, a recent study uncovers serious flaws in the pesticide registration process at the U.S. Environmental Protection Agency (EPA) with an in-depth evaluation of the agency’s failure to protect the public from the harmful effects of five neonicotinoid (neonic) insecticides—as mandated by the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) and amendments, including Food Quality Protection Act (FQPA) of 1996. This coincides with EPA’s ongoing review to renew their approval for the next 15 years (set to be announced in 2025). The report is based on the first comprehensive assessment of unpublished rodent-based Developmental Neurotoxicity (DNT) studies, conducted between 2000-2003 and submitted by pesticide manufacturers as part of the registration process. All five neonicotinoids evaluated—acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam—are associated with significant shrinkage of brain tissue at the highest dosage, according to EPA data reports (see acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam). However, with little or no data regarding the chemicals’ impacts at low and mid-level dosages, EPA has either failed to find a “No Observed Adverse Effect Level” (NOAEL) or, seemingly at random, set the NOAEL at the mid-level dosage. The evaluation suggests that perinatal exposure to […]

(Beyond Pesticides, April 30, 2024) Researchers found epigenetic changes, including changes relating to “DNA methylation, histone modification, and differential microRNA expression [which ‘can alter the expression of many disease-related genes’],” in a systematic review and meta-analysis of existing literature published in Environmental Epigenetics. “Our review did provide evidence that pesticide exposure could lead to epigenetic modifications, possibly altering global and gene-specific methylation levels, epigenome-wide methylation, and micro-RNA differential expression,” researchers share in the conclusion of the study. This study is an amalgamation of various studies on epigenetic changes based on a literature review process: “Article review involved [3,529 articles found through] extensive searches across major human health databases, including PubMed, Embase, and Cochrane, and BVS (Biblioteca Virtual em Saúde – the Latin American Health Database). Searches covered articles published through December 2020. Considering the diverse terminologies used to describe the same epigenetic mechanism in this field, the search strategy aimed to encompass all relevant articles by combining a variety of search terms in titles and abstracts. This approach was implemented across PubMed, Embase and Cochrane databases to ensure comprehensive coverage.” Studies were not included if the participants were not considered “healthy individuals” or if the participants had “known inherent/congenital or […]

(Beyond Pesticides, January 10, 2024) Exposure to endocrine-disrupting chemicals (EDCs) negatively impacts testicular function and may cause sperm count declines over time, according to a 2022 review published in Endocrine. The findings indicate that this occurs regardless of whether exposure is prenatal (before birth) or postnatal (after birth). More recent work from October 2023 confirms the connection between male reproductive health and exposure to organophosphate and carbamate insecticides and the weed killer glyphosate—as many pesticide products containing these chemicals are classifiable as endocrine disruptors (ED). Just last year, a meta-analysis from researchers at Mount Sinai Medical Center, the Hebrew University of Jerusalem, and the University of Copenhagen, among others, finds that the drop in global sperm count is accelerating, dropping by 51.6 percent from 1973 through 2018. The U.S. regulatory system, under the authority of the U.S. Environmental Protection Agency (EPA), has not kept pace with the science and does not fully evaluate pesticides in wide use for endocrine disruption, despite a requirement in 1996 law (the Food Quality Protection Act) to begin that testing and evaluation nearly three decades ago. In 2021, Beyond Pesticides reported that the Office of the Inspector General (OIG) for EPA issued a damning report on the […]

(Beyond Pesticides, January 3, 2024) A study published in Environmental Health Perspectives finds elevated, chronic exposure to glyphosate throughout one’s lifetime increases the risk of mosaic loss of chromosome Y (loss of chromosome Y occurs to many men in some cells due to aging [mLOY]) that impacts a noticeable fraction of cells. Although the loss of this sex chromosome does not cause cell death, like the loss of autosomal chromosomes, the risk of mLOY is a biomarker for genotoxicity (the damage of genetic information within a cell causing mutations from chemical exposure, which may lead to cancer) and expansion of cellular response to glyphosate, resulting in the precursor for hematological (blood) cancers. This study is one of the first to identify sex-specific chromosome degradation, with stark evidence demonstrating links to various cancers, including non-Hodgkin lymphoma. The International Agency for Research on Cancer (IARC) has classified the glyphosate as a probable carcinogen or cancer-causing chemical. However, the U.S. Environmental Protection Agency’s (EPA) allowance of widespread use of glyphosate allows for adverse impacts, especially among vulnerable individuals, like pregnant women, infants, children, and the elderly. Glyphosate exposure levels and resulting residues in urine has been documented with recent data showing that four out of five (81.6%) […]

(Beyond Pesticides, October 12, 2023) A new study published in Environment International finds novel environmental chemicals (i.e., piperidine insecticide, 2,4-dinitrophenol, benzo[a]carbazole, and a benzoate derivative) involved in developing breast cancer through various inflammation pathways. These new potential factors contribute to breast cancer and highlight the importance of employing epidemiological biomonitoring like exposome (total exposure from birth to death) to discover mechanisms involved in disease development that are otherwise overlooked. According to the Centers for Disease Control and Prevention (CDC), breast cancer is a disease that causes breast cells to grow out of control, with the type of breast cancer depending on the cells themselves. Several studies and reports, including U.S. Environmental Protection Agency (EPA) data, identify hundreds of chemicals as influential factors (either promoting or initiating) associated with breast cancer risk. Breast cancer is the most common cancer among women, accounting for 12 percent of all new annual cancer cases worldwide and causing the second most cancer-related deaths in the United States. Past studies suggest genetic inheritance factors influence breast cancer occurrence. However, genetic factors only play a minor role in the incidence of breast cancer, while exposure to external environmental factors (e.g., chemical exposure) appears to play a more notable role. One in […]

(Beyond Pesticides, August 23, 2023) Researchers are investigating a cutting-edge method to identify the impact of pesticides on reproductive health—shrinking the wait time from months to weeks. Scientists at the University of California, Davis, are developing a method for identifying harmful chemicals in pesticides with the help of glowing fish. This scientific breakthrough could revolutionize pesticide research and help prevent long-term health problems caused by exposure to these chemicals.  Pesticide exposure can cause acute and long-term health problems for the human endocrine system, the hormone system that regulates many biological processes from reproduction to blood sugar, growth, and more. Beyond Pesticides has written about the connections between EPA-registered pesticides and involuntary abortions, reproductive cancers, pregnancy loss, early-onset puberty, and more.   The American Academy of Pediatrics (AAP) has expressed concern over the limited or missing data regarding the health effects of pesticides and food additives on infants and children, who are more vulnerable to chemical exposures. AAP has identified several compounds as being of particular concern, including bisphenols, which are commonly used in the lining of metal cans; phthalates, which are used in adhesives and plasticizers; nonpersistent pesticides, which have been addressed in a previous AAP policy statement; perfluoroalkyl chemicals (PFCs), […]

(Beyond Pesticides, August 11, 2023) Reinforcing earlier findings, a systematic review published in Chemosphere finds the popular herbicide glyphosate and its formulations (glyphosate-based formulations-GBF) exhibit five out of the ten key characteristics (KC) of carcinogens (cancer-causing chemicals). Specifically, glyphosate exhibits strong evidence of genotoxicity, epigenetic alterations (heritable changes in gene expression), oxidative stress, chronic inflammation, endocrine disruption, and disturbs gut microbiota implicated in lymphomagenesis (growth and development of lymphoma). Although organizations like the International Agency for Research on Cancer (IARC) designate glyphosate as a probable human carcinogen, and scientific literature supports the findings on these adverse effects purported by glyphosate, the chemical remains on the U.S. market in various formulations. Glyphosate is the most commonly used active ingredient worldwide, appearing in many herbicide formulas, not just Bayer’s (formerly Monsanto) Roundup®. The use of this chemical has been increasing since the inception of crops genetically modified to tolerate glyphosate over two decades ago. The toxic herbicide readily contaminates the ecosystem with residues pervasive in food and water commodities. In addition to this study, literature proves time and time again that glyphosate has an association with cancer development, as well as human, biotic, and ecosystem harm. Therefore, advocates point to the need for national policies to reassess hazards associated with […]

(Beyond Pesticides, September 8, 2022) A study published in Environmental Pollution finds parental exposure to real-world, sublethal concentrations of pesticide residues on grains is a major contributor to unfavorable offspring development among foraging birds. Parents’ ingestion of grains with conventional pesticide residues, whether from contaminated or pesticide-treated seeds, results in chronic exposure that adversely affects offspring health, even at low doses. The adverse effects pesticides and other environmental pollutants have on birds are amply documented and researched. Although many studies evaluate acute or chronic health implications associated with pesticide exposure in a single generation, there is a lack of information on multi-generational impacts that can provide vital information on the fundamental survivability or fitness of bird species. Considering this study emphasizes parental exposure to environmental pollutants can have adverse consequences for future generations, it is necessary that future risk assessments for birds address these implications when implementing agricultural pesticide policies. The study notes, “[S]ublethal effects of such compounds [pesticides] on non-target species should be included in the regulation. Moreover, as agroecosystem pollution is not resulting only from pesticides, there is an urgent need to analyze cocktail effects, not only between molecules of pesticides but also between pesticides and other pollutants such as […]

(Beyond Pesticides, March 10, 2022) Glyphosate and glyphosate-based herbicides (GBH) like Roundup® induce DNA damage and alter biological mechanisms (gene regulatory microRNAs [miRNAs or miRs]) associated with cancer development. According to the study published in Toxicological Sciences, DNA damage mainly occurs through oxidative stress from GBH exposure. Moreover, DNA damage and other biological mechanisms that cause carcinogenicity (cancer) occur at doses assumed “safe” by pesticide regulators such as the U.S. Environmental Protection Agency (EPA). Glyphosate is the most commonly used active ingredient worldwide, appearing in many herbicide formulas, not just Bayer’s (formerly Monsanto) Roundup®. The use of this chemical has been increasing since the inception of crops genetically modified to tolerate glyphosate over two decades ago. The toxic herbicide readily contaminates the ecosystem with residues pervasive in food and water commodities. In addition to this study, literature proves time and time again that glyphosate has an association with cancer development, as well as human, biotic, and ecosystem harm.  Study lead author Michael Antoniou, Ph.D., cautions, “Our results are the first to simultaneously show glyphosate and Roundup toxicity in a whole mammalian animal model system and provide a mechanism – oxidative stress – by which DNA damage has been observed in other systems, such as mammalian […]

(Beyond Pesticides, April 22, 2021) Past maternal exposure to the pesticide dichlorodiphenyltrichloroethane (DDT) during pregnancy can increase the risk of breast cancer and cardiometabolic disorders (e.g., heart disease, obesity, diabetes) up to three successive generations, according to a new study published in Cancer Epidemiology, Biomarkers & Prevention. Although previous studies highlight early life or in utero exposure to DDT increasing breast cancer risk later in life, this study is the first to note generational effects on grandchildren’s health. DDT continues to adversely affect the health of the U.S. population, nearly 50 years after its ban. However, this ban is not global, as many countries still use or manufacture the chemical compound. Furthermore, residues of DDT metabolite, DDE, continue to readily contaminate food and water worldwide. Therefore, studies like these highlight the need to investigate how first-generation pesticide exposure can impact future generational health in order to prevent adverse health outcomes, especially during sensitive developmental periods (i.e., in utero, infancy/childhood). The study researchers note, “Discovery of actionable biomarkers of response to ancestral environmental exposures in young women may provide opportunities for breast cancer prevention.” To assess the association between multi-generational health risks and chemical exposure, researchers used the Public Health Institute’s Child […]

(Beyond Pesticides, July 2, 2020) Exposure to low levels of endocrine-disrupting chemicals commonly in waterways, including pesticides, can impact future generations of major commercial fish, despite no direct exposure to the chemicals, according to research published in the journal Frontiers in Marine Science by Oregon State University (OSU) researchers. Many studies assess the acute or chronic health implications associated with endocrine disruptors on a single generation but lack information on multi-generational impacts that can provide vital information on the fundamental survivability or fitness of many species. This study highlights the significance of understanding the implications of endocrine disruptors, even at low levels of exposure, as parental exposure can have adverse epigenetic consequences for future generations. Kaley Major, a Ph.D. fellow at Oregon State University (OSU) and lead research author, explains, “What t[his] gets at is something your grandparents may have come into contact within their environment can still be affecting the overall structure of your DNA in your life today.” Endocrine disruptors are xenobiotics (i.e., chemical substances like toxic pesticides foreign to an organism or ecosystem). Past research shows exposures to endocrine-disrupting chemicals can adversely impact human, animal—and thus environmental—health, by altering the natural hormones in the body responsible for […]

(Beyond Pesticides, February 13, 2020) Congress unanimously passed the Food Quality Protection Act (FQPA) in 1996 to increase protections for children from pesticide exposure. Unfortunately, according to a new study published in Environmental Health, the law is not being employed by the Environmental Protection Agency (EPA) to its full capacity. For most of the 59 pesticides reviewed by the study, EPA did not apply an additional FQPA safety factor and thereby missed an opportunity to protect children’s health. In fact, FQPA solidified EPA’s reliance on risk assessment calculations and mitigation measures that consistently fall short of adequate levels of protection because of serious data gaps, a failure to consider exposure to mixtures and synergistic effects, and a bias against consideration of alternatives (alternatives assessment)  that show toxic pesticides to be unnecessary.  FQPA establishes a safety standard applied to all food commodities that considers specific risks for infants and children. The law requires EPA to assess the “aggregate risk” (considering exposure from multiple sources) and “cumulative exposure” to pesticides that have a “common mechanism of toxicity.” FQPA mandates “an additional tenfold margin of safety for the pesticide chemical residue and other sources of exposure shall be applied for infants and children […]