(Beyond Pesticides, October 23, 2024) The stark contrast of two political parties emerged around this summer’s reporting of the Project 2025 blueprint—created by extreme right-wing conservatives—that proposes the gutting of environmental and public health policy and implementation. Many political observers say “Project 2025 Presidential Transition Project,” formally titled “Mandate for Leadership: The Conservative Promise,” will be embraced by a second Trump Administration, despite denials that are challenged by insiders as outright lies. While the public became aware of Project 2025 plans to gut the U.S. Environmental Protection Agency (EPA) and many other agencies, the Biden Administration was announcing the emergency ban (see also August 6 announcement), finalized yesterday, of the weed killer Dacthal, exercising an EPA authority that has not been used in 45 years since the banning of 2,4,5-T (50% of the mixture of Agent Orange). With this decision, EPA set an important precedent for proclaiming (i) an unacceptable harm, (ii) its inability to mitigate the pesticide’s hazards with typical risk mitigation measures, and (iii) the availability of alternatives that made the chemical unnecessary. In dramatic contrast, the Trump supporters behind Project 2025 are intent on politicizing science to undermine governmental structures and laws established to protect public health […]

Beyond Pesticides (October 17, 2024) On September 30, 2024, the U.S. Environmental Protection Agency (EPA) opened a public comment period about production of specific per- and polyfluoroalkyl substances (PFAS, also known as ‘forever chemicals’)—including perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA). EPA is collecting information on the fluorination process of high-density polyethylene (HDPE) and other plastic containers to inform possible regulatory action under the Toxic Substances Control Act (TSCA). The deadline for submitting comments is November 29, 2024. PFOA and twelve other PFAS compounds are created during the fluorination of HDPE plastic containers by Inhance Technologies, LLC, the only U.S. company manufacturing containers using this fluorination technique (see here). Studies by EPA, independent researchers, and the company itself demonstrate that PFAS leaches from container walls into contents, exposing millions to these toxic chemicals without their knowledge. EPA notes, “Long-chain PFAS like PFOA, PFNA, and PFDA build up in our bodies and the environment over time. Even small amounts can significantly contribute to people’s long-term exposure and health risk for cancers, impacts to the liver and heart, and immune and developmental damage to infants and children.” The adverse effects of PFAS exposure are linked to serious health issues, […]

(Beyond Pesticides, October 11-14, 2024) Indigenous Peoples’ Day is celebrated this year on Monday, October 14, 2024 and has been described as follows: “[T]hroughout our Nation’s history, Indigenous peoples have faced violence and devastation that has tested their limits. . . Today, Indigenous peoples are a beacon of resilience, strength, and perseverance as well as a source of incredible contributions. . . They challenge all of us to celebrate the good, confront the bad, and tell the whole truth of our history.  And as innovators, educators, engineers, scientists, artists, and leaders in every sector of society, Indigenous peoples contribute to our shared prosperity.  Their diverse cultures and communities today are a testament to the unshakable and unbreakable commitment of many generations to preserve their cultures, identities, and rights to self-governance.  That is why, despite centuries of devastation and turmoil, Tribal Nations continue to thrive and lead in countless ways.” This language is taken from a Proclamation issued by President Biden (2023), first issued in 2021 and then again last year. This year’s Proclamation states: “On Indigenous Peoples’ Day, we honor Indigenous peoples’ strength, courage, and resilience.  We celebrate the vast contributions of Indigenous communities to the world.  And we recommit […]

(Beyond Pesticides, September 25, 2024) In a semiannual report released in August 2024, EPA’s Office of Inspector General (OIG) identifies a number of “unresolved” issues that strike the core of the agency’s failure to carry out its responsibilities to protect health and the environment. One of the issues identified is EPA’s failure to conduct an adequate and independent assessment of the cancer effects of the fumigant, 1,3-Dichloropropene (1,3-D or Telone). OIG’s original report, The EPA Needs to Improve the Transparency of Its Cancer-Assessment Process for Pesticides, was issued in 2022 and concluded that EPA’s Office of Pesticide Programs (OPP) engages in secret meetings with industry, elevates unqualified individuals to decision-making roles, uses an untested scientific approach, fails to conduct a simple literature review, and neglects public transparency. Other pesticide issues that OIG identified in its report include: The EPA Needs to Determine Whether Seresto Pet Collars Pose an Unreasonable Risk to Pet Health The EPA Has Not Verified that Its Laboratories Comply with Hazardous Waste Requirements EPA Needs an Agencywide Strategic Action Plan to Address Harmful Algal Blooms EPA’s Endocrine Disruptor Screening Program Has Made Limited Progress in Assessing Pesticides EPA Needs to Evaluate the Impact of the Revised Agricultural Worker […]

(Beyond Pesticides, September 24, 2024) Recent commentary in Frontiers in Toxicology by two researchers, Maricel Maffini, PhD and Laura Vandenberg, PhD, highlights the pitfalls in the current regulatory systems in the United States (U.S.) for chemicals that threaten human health. Despite many advancements in science over the past few decades, and the wealth of studies that tie chemical exposure to negative health effects, risk assessments, and subsequent risk management, remain “static” and “outdated,” according to the authors. “There is increasing concern amongst public health professionals, environmental health scientists, and medical organizations about exposures to synthetic chemicals,” the researchers say. “These organizations’ concerns are based on the overwhelming evidence showing associations between chemical exposures and adverse health outcomes in human populations.” Such concerns have sparked a debate on current regulatory methods for chemicals that are present in highly used products, such as pesticides, plastic containers, and food.    The authors continue, “There are now thousands of studies showing associations between these chemicals and adverse health effects in humans including neurological disorders and learning disabilities, metabolic outcomes, infertility, thyroid dysfunction, and cancers.” Additional health effects can be seen in the Pesticide-Induced Diseases Database. Of the many harmful chemicals, per- and polyfluoroalkyl substances […]

(Beyond Pesticides, September 20, 2024) As the California State Assembly wrapped up the 2024 legislative session, what was once a proposed ban of the deadly weed killer paraquat (in both agricultural and nonagricultural contexts) was amended and passed as a requirement for California Department of Pesticide Regulation to complete an “expedited review” by January 1, 2029. Paraquat exposure has been linked to Parkinson’s disease. According to reporting by Los Angeles Times, Assemblymember Laura Friedman (D-Burbank) (the original sponsor of the bill) spoke candidly about the prospects for this legislation, “We never thought we’d get a full ban through the Legislature. But we had to push as hard as we could.” Assemblymember Friedman is chair of the bicameral Environmental Caucus and a self-described “steadfast advocate for the environment [and] sustainable communities.” Environmental advocates, public health professionals, and many who have been affected by Parkinson’s disease are calling for the emergency suspension of paraquat, applying the same standard used by the U.S. Environmental Protection Agency (EPA) to ban the herbicide Dacthal/DCPA last month. (See Daily News here.) Context The original legislation responds to significant scientific documentation of the pesticide’s hazards, and an unresponsive regulatory process, as well as previous legislation efforts. In […]

(Beyond Pesticides, September 18, 2024) A literature review in Trends in Analytical Chemistry analyzes scientific articles from the last ten years from around the globe that identify more than 300 pesticides in bee pollen. Bee pollen, often used as an edible dietary supplement, is not regulated for pesticide residues, which sparks concern for human exposure due to contamination with pesticides, heavy metals, metalloids, and mycotoxins. “Bee pollen is a food supplement that is receiving increasing attention for its nutraceutical and therapeutic properties. However, several uncertainties on the safety of this beekeeping product still exist. The present work addressed this issue through the critical evaluation of 61 studies, published over the 2014–2024 period,” the Spanish authors state. Bee pollen is produced by honey bees. After they forage on flowers and gather pollen on their hind legs (in pollen baskets or corbiculae) to transport back to the hive, it is moistened with nectar and salivary secretions to create bee pollen in the form of pellets. While the composition of bee pollen can vary between geographical locations with different flowers, the studies reviewed all utilize mass spectrometry to pinpoint pesticides, as well as mycotoxins (created by naturally occurring mold spores), that threaten human […]

(Beyond Pesticides, August 30, 2024) There is no more compelling reason to embrace a precautionary pesticide poisoning standard this Labor Day than the need to protect workers. In fact, the Occupational Safety and Health Administration (OSHA) says on its website, Transitioning to Safer Chemicals, that the best way to protect workers is to  “eliminate or reduce hazardous chemicals at the source.” While some try to employ product substitution with “safer” chemicals, Beyond Pesticides urges decision makers to embrace alternative systems, such as organic management systems, that embrace management techniques to meet disease and infestation management goals and use only organic compatible substances. According to OSHA: “In chemical management, [the industrial hygiene principle, known as the hierarchy of controls] guides employers and workers to eliminate or reduce hazardous chemicals at the source by substituting them with safer alternatives. Unlike traditional engineering controls, administrative controls, work practice controls, or personal protective equipment, these strategies can completely eliminate exposure to hazardous chemicals, reduce the potential for chemical accidents, reduce disposal costs, and remove concerns regarding worker compliance and equipment maintenance.” A look through the state and federal databases that track occupational pesticide poisoning for both acute (immediate short-term) and chronic (long-term) pesticide effects […]

(Beyond Pesticides, August 28, 2024) The U.S. Environmental Protection Agency’s (EPA) pesticide labeling requirements fail to adequately communicate acute toxicity levels to the public, as evidenced in a recent study of consumers published last month in the journal Nature. After evaluating whether the current three “signal” words (CAUTION, WARNING, DANGER) on pesticide products adequately convey pesticide toxicity, the authors conclude that current labeling may result in “unintended adverse effects” because it does not “effectively communicate toxicity risks to consumers.” The signal words on pesticide labels, based on laboratory animal testing for determining lethal doses, are intended to protect users of the product from exposure that can kill through inhalation, skin absorption, and ingestion of the pesticide. However, the signal words do not warn about long-effects like cancer, neurological diseases, reproductive harm, as well as other adverse effects associated with pesticide exposure. (See Pesticide-Induced Diseases Database.)   The study tests two prototype labels to evaluate the effectiveness of visual elements in communicating toxicity information, citing research in cognitive psychology that indicates visual elements, like images and graphics, are more effective for conveying information than text alone. This is particularly crucial for pesticide labels, where complex toxicity details need to be communicated quickly […]

(Beyond Pesticides, August 26, 2024) In July, the U.S. Environmental Protection Agency (EPA) announced it was raising the allowable levels of the highly toxic weed killer atrazine in the nation’s waterways from the 2016 level of 3.4 to 9.7 micrograms per liter (µg/L), which scientists and environmental advocates say is a serious threat to aquatic plants, fish, invertebrates, and amphibians, in addition to people who recreate in waterways or eat food from them. With EPA’s August 7 decision to ban the weed killer Dacthal (or DCPA–dimethyl tetrachloroterephthalate), Beyond Pesticides is rallying public support for the removal of atrazine from the market under the same standards of harm, inability to mitigate hazards, and the availability of alternatives. As Beyond Pesticides points out in its 2022 atrazine comments (2020 and 2016 comments included) to EPA, the agency in November 2021 released the final Biological Evaluation (BE) assessing risks to listed species from labeled uses of atrazine (in the triazine chemical family). The agency made “likely to adversely affect (LAA) determinations” for 1,013 species and 328 critical habitats, which it is now rejecting, while using a “community-equivalent level of concern (CE-LOC)” measure that is filled with uncertainty and lacks any sense of precaution with […]

Image: Art Page submission from Sara Grantham, “Sunflower Pollinators”  (Beyond Pesticides, August 23, 2024) A study in Science of The Total Environment calculates and compares pesticide risk in 594 wild bee species associated with crops in North America. Current pesticide risk assessments that analyze effects on bees primarily focus on a limited subset of species and do not provide comprehensive protection of all wild bees. “Species commonly proposed as models for pesticide risk assessments may not accurately represent risk for those bee species facing the highest potential risk in agricultural contexts,” the authors postulate. The researchers continue, “This study presents a novel approach to characterize and compare the relative potential pesticide risk among wild bee species of their association with crops in North America using suites of intrinsic bee traits to quantify species’ vulnerability and extrinsic factors based on the toxic load of crops for bees and the strength of each species’ association with those crops.” In considering multiple factors that vary by species and determining potential harm to each from pesticide exposure, this study highlights the inadequacies of the current risk assessment process used by the U.S. Environmental Protection Agency (EPA).    The system for risk assessment for pesticides that […]

(Beyond Pesticides, August 20, 2024) A study published online in the journal Environmental Toxicology and Pharmacology raises continuing concern about residual exposure to organochlorine pesticides (OCPs) and the disruption that they and their metabolites and isomers cause to biological systems. For the most part, OCPs, including dichlorodiphenyltrichloroethane (DDT), are no longer used worldwide, but the legacy of their poisoning and contamination persists. A 2022 article in Environmental Science & Technology cites California condors and marine mammals along California’s coast contaminated with several dozen different halogenated organic compounds (hazardous, often-chlorinated chemicals) related to DDT, chlordane, and other now-banned legacy chemicals. Other research finds DDT in deep ocean sediment and biota. And, more research finds multigeneration effects from DDT exposure with grandmothers’ exposure to DDT increasing granddaughters’ breast cancer and cardiometabolic disorder risk. This study may be the first compilation of research regarding the modes of action for distinct types of organochlorine pesticides (OCPs). The findings raise the significant danger of legacy chemicals that persist for generations and call for a precautionary regulatory standard that is focused on preventing harm and advancing alternative nontoxic practices and products. In tracking the ongoing scientific literature on a broad spectrum of adverse effects daily, Beyond Pesticides […]

(Beyond Pesticides, August 12, 2024)  When the U.S. Environmental Protection Agency (EPA) issued an emergency ban of the weed killer Dacthal (DCPA) last week, it said that there are no “practicable mitigation measures” to protect against identified hazards—a clear and honest assessment of the limits of pesticide product label changes and use restrictions. Now, the question is whether the same thinking can be applied across the EPA’s pesticide program, addressing the urgent need to protect biodiversity. In the Dacthal proclamation, EPA said it consulted with the U.S. Department of Agriculture (USDA) on “alternatives to this pesticide,” and presumably determined that there were “alternative chemicals” that could be used in chemical-intensive agriculture—while not considering “alternatives to chemicals.” This is the framework that is understood to be EPA’s process that keeps pest management on a pesticide treadmill except in extremely rare cases (this being the second in nearly 40 years). It is also the framework that has led to catastrophic events or existential crises on biodiversity collapse, health threats, and the climate emergency. On biodiversity, the mix of diverse and intricate relationships of organisms in nature that are essential to the sustaining of life, EPA’s pesticide program, the Office of Pesticide Programs, has […]

(Beyond Pesticides, August 8, 2024) With the use of its emergency authority—not used in nearly 40 years, the U.S. Environmental Protection Agency (EPA) on August 7 banned a pesticide (the weed killer Dacthal or DCPA—dimethyl tetrachloroterephthalate) under the “imminent hazard” clause of the federal pesticide law. At the same time, the agency is exercising its authority to prohibit the continued use of Dacthal’s existing stocks, a provision that EPA rarely uses. EPA identified serious concerns about fetal hormone disruption and resulting “low birth weight and irreversible and life-long impacts to children [impaired brain development, decreased IQ, and impaired motor skills] exposed in-utero” and finds that there are no “practicable mitigation measures” to protect against these hazards. The last time EPA issued an emergency action like this was in 1979 when the agency acknowledged miscarriages associated with the forestry use of the herbicide 2,4,5-T—one-half of the chemical weed killer Agent Orange, sprayed over people to defoliate the landscape of Vietnam in the war there—with the most potent form of dioxin, TCDD (2,3,7,8-Tetrachlorodibenzo-p-dioxin). While EPA has been congratulated for using its emergency authority, which it is obviously reluctant to use, and health and environmental activists say could be used broadly, the timeline […]

(Beyond Pesticides, August 5, 2024) As the longstanding problem of involuntary pesticide exposure through chemical drift continues unabated, EPA announced “new, earlier protections” that are being criticized by Beyond Pesticides as inadequate. The organization is calling on the public to let EPA and Congress know that it must comprehensively eliminate nontarget chemical trespass. Beyond Pesticides notes that the recent EPA announcement does not stop the movement of pesticides off the target sites restricted by pesticide product labels and therefore does not protect the public and environment in agricultural, rural, suburban, and urban areas from resulting health and ecological threats. EPA’s July 15, 2024, press release, “EPA Announces New, Earlier Protections for People from Pesticide Spray Drift” states, “The Agency is not making any changes to its chemical-specific methodology outlined in the 2014 document but has decided to extend the chemical-specific spray drift methodology to certain registration actions.” Although EPA should evaluate every pesticide use for its drift potential, extension of an inadequate process does not constitute “protection.” >> Tell EPA and Congress that EPA must protect against all forms of pesticide drift. Pesticide drift—more properly designated “chemical trespass”—is a threat to people living in agricultural, rural, suburban, and urban areas, […]

(Beyond Pesticides, August 2, 2024) In a recent study published in Science, a team from the University of Massachusetts and Yale University provides quantitative insight into the significant effects of a recent U.S. Supreme Court decision on the nation’s water quality. This research highlights the essential role of ephemeral streams—water sources that flow temporarily after rainfall—in transporting pollutants, including pesticides, sediments, and nutrients from land to larger water bodies.  This comprehensive study underscores the devastating risk to U.S. water quality, stemming from the May 2023 U.S. Supreme Court decision, Sackett v. Environmental Protection Agency (EPA), which dramatically limits the agency’s ability to protect ephemeral streams as well as critical wetland ecosystems under the Clean Water Act (CWA).  As a May 2024 report by Clean Water for All Coalition notes, “The [Sackett] decision has endangered the drinking water sources of at least 117 million Americans by stripping protections from over half of the nation’s wetlands, as well as up to nearly 5 million miles of rain-dependent and seasonal streams that feed into rivers, lakes, and estuaries.” At a time when an immediate response to the climate crisis and chemical pollution is more urgent than ever, the U.S. Supreme Court’s judicial decisions are seen […]

(Beyond Pesticides, July 31, 2024) The latest commentary on “forever chemicals” in Environmental Health Perspectives captures growing concerns for the class of per- and polyfluoroalkyl substances (PFAS) that are found in pesticide products and cause persistent contamination that threaten human health and the environment. The authors share, “Given that pesticides are some of the most widely distributed pollutants across the world, the legacy impacts of PFAS addition into pesticide products could be widespread and have wide-ranging implications on agriculture and food and water contamination.” Fluorination, which adds fluorine to a compound, is used to modify properties, such as the stability of chemicals. It can also increase residual activity of pesticide ingredients. Fluorinated molecules, including PFAS, are “a serious environmental health concern owing to their highly persistent nature, often potent toxicities, potential to bioaccumulate, and widespread presence in people, animals, and the broader environment,” the authors state. They continue in saying, “The long-term impacts of using mixtures of extremely persistent chemicals on potentially hundreds of millions of acres of US land every year is, to us, a cause for concern.” The commentary, titled “Forever Pesticides: A Growing Source of PFAS Contamination in the Environment,” explores how and to what extent PFAS […]

(Beyond Pesticides, July 30, 2024) The U.S. Environmental Protection Agency (EPA) announced on July 15 what it described as a new process for evaluating the risks of spray drift—the migration of pesticides from their target area to off-site zones. According to a statement by EPA Chemical Safety and Pollution Prevention Assistant Administrator Michal Freedhoff, PhD in an Oregon Public Broadcasting story, the agency took the step so that “people don’t have to wait years for the protections they deserve and need.” However, EPA states, “The Agency is not making any changes to its chemical-specific methodology outlined in [its] 2014 document but has decided to extend the chemical-specific spray drift methodology to certain registration actions.” EPA has said, “Spray drift is governed by a variety of factors which govern how much of the pesticide application deposits on surfaces where contact with residues can eventually lead to indirect exposures (e.g., children playing on lawns that are next to treated fields and where residues have deposited).” The new policy will add spray drift evaluation to occasions when the agency receives an application for a new pesticide and when a registered pesticide is intended for a new use or applied to a new crop. […]

(Beyond Pesticides, July 29, 2024) Beyond Pesticides is asking the public to support a petition to the U.S. Environmental Protection Agency (EPA), filed by environmental and farm groups, to require the agency’s pesticide registration program to ban PFAS (so-called “forever chemicals”) as pesticide ingredients and all their uses that result in contamination of pesticide products. The petition, submitted by the Center for Food Safety (CFS) on behalf of 12 other petitioners, asks EPA to: “(1) ban PFAS as pesticide ingredients; (2) adopt a broad definition of PFAS chemicals that reflect the current scientific understanding of the class of chemicals; (3) prohibit the use of PFAS-containing containers for pesticide storage; (4) mandate reporting of PFAS contamination from pesticide registrants; and (5) prevent future contamination by requiring pesticide manufacturers to submit data specific to PFAS before future registrations can be approved.” PFAS—per- and polyfluorinated substances—is a group of nearly 10,000 highly persistent, human-made toxic chemicals. A commentary released last week in Environmental Health Perspectives, Forever Pesticides: A Growing Source of PFAS Contamination in the Environment, “explore[s] different ways that PFAS can be introduced into pesticide products, the extent of PFAS contamination of pesticide products, and the implications this could have for human […]

(Beyond Pesticides, July 26, 2024) Beyond Pesticides released the latest issue of Pesticides and You this week, a compendium of scientific research on pesticide threats to human and environmental health. The issue is a breathtaking warning from the science community that environmental, health, and labor laws are not protecting the public. Beyond Pesticides says in its introduction that a shift away from toxic pesticide use is urgently needed. Included in this issue are scientific reviews of research reported by Beyond Pesticides in 2023, providing a critique of the independent peer-reviewed literature with a shocking range of adverse effects, including cancer, neurotoxicity, brain effects, reproductive impacts, diabetes and obesity, chronic kidney and liver disease, Parkinson’s, respiratory illness and asthma, learning and behavioral abnormalities, and more, as well as disproportionate harm to people of color. In addition, the science documents pesticides’ catastrophic harm to the ecosystems that sustain life. In total, these dramatic findings call for an end to the use of toxic pesticides, incompatible with respect for living organisms and, to environmental, health, and labor advocates, unconscionable given the availability of viable, cost-effective organic practices. This issue adds to the body of knowledge from two previous issues of Pesticides and You (Transformative Change: […]

(Beyond Pesticides, July 25, 2024) On July 10, the Oregon Court of Appeals ruled that the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA) does not preempt pesticide exposure victims’ state law claims against pesticide manufacturers, based on reporting from The New Lede. This decision builds on years of judicial precedent from the Supreme Court of the United States (SCOTUS) that protects individuals’ right to use failure-to-warn claims against producers of toxic pesticides, including Bayer-Monsanto. The importance of judicial review is critical to protecting the public against public health impacts of toxic pesticide use in the context of last month’s SCOTUS decision ending Chevron Doctrine, and with it the end of deferring to federal regulatory agencies on ambiguities in statutory mandates. A growing coalition of environmental and public health advocates, organic farmers, trial attorneys, farmworkers, and physicians are united in pushing back against a concerted effort by industry and its allies to attack victims’ ability to sue under “failure-to-warn” through the Farm Bill, state legislatures, and the proposed federal budget for Fiscal Year 2025. Oregon Court of Appeals In 2022, a local trial court in Oregon ruled in favor of Monsanto on a lawsuit initiated by Jackson County residents Larry and […]

(Beyond Pesticides, July 24, 2024) The Center for Food Safety—joined by environmental, farm, and grassroots organizations including Beyond Pesticides—submitted a groundbreaking petition yesterday to the U.S. Environmental Protection Agency (EPA), urging immediate action to address the presence of per- and polyfluoroalkyl substances (PFAS) in pesticides and pesticide containers. Numerous studies have shown that the broad use of PFAS chemicals, and the resulting environmental contamination, has devastating impacts on public health, wildlife, and pollinators. Despite acknowledging PFAS as an urgent public health and environmental issue, EPA has upheld hundreds of registrations of pesticide ingredients that fall into the PFAS category. Furthermore, EPA has allowed the ongoing use of fluorinated pesticide storage containers, despite the agency itself finding that these containers leach PFAS chemicals into pesticide products.   PFAS, known as “forever chemicals” due to their ability to persist in the environment, are endocrine disruptors, which are linked to developmental issues, cancers, and organ damage. Crops can uptake PFAS from soil, resulting in dietary exposure for both the public and wildlife, while PFAS ingredients in pesticides can also leach into groundwater, contributing to widespread PFAS drinking water contamination. In recent years, EPA has acknowledged this critical issue and committed to addressing PFAS contamination outside […]

(Beyond Pesticides, July 18, 2024) A recent study in The Journal of Toxicological Sciences shows that a single dose of the neonicotinoid insecticide clothianidin (CLO) induces behavioral abnormalities, predominantly in female mice, throughout key stages of development. In testing mice at various ages, sex-specific changes were identified that highlight not only varied effects on males and females but also how pesticide exposure at a young age can cause lasting impacts throughout adulthood in mammalian species.  The researchers, at the Laboratory of Animal Reproduction and Development at Tohoku University in Japan, “utilized murine [mouse] models to compare the sex-specific differences in behavioral effects following CLO exposure at different developmental stages. [They] orally administered CLO to male and female mice as a single high-dose solution (80 mg/kg) during the postnatal period (2-week-old), adolescence (6-week-old), or maturity (10-week-old), and subsequently evaluated higher brain function.”   As the authors remark, “Most studies on the neurotoxicity of CLO have targeted only males, with limited insights regarding the neurodevelopmental toxicity in females. There are significant sex differences in brain development due to hormonal, genetic, epigenetic, and other sex-specific factors. Moreover, there are also a number of sex-based differences in the prevalence of developmental disorders, such as […]