(Beyond Pesticides, April, 29, 2014) According to a recent study published in the American Chemical Society’s (ACS) journal Chemical Research in Toxicology, the chemicals triclosan and octylphenol are linked to the growth of breast cancer cells. Triclosan is an antibacterial and antifungal agent found in numerous commonly known household products. Octylphenol is a commercial solvent that can be found in paints and plastics, and is often used as an inert ingredient in pesticide formulations. Researchers investigated whether these two endocrine-disrupting chemicals (ECDs) contributed to the growth of cancer cells. In their study, Progression of Breast Cancer Cells Was Enhanced by Endocrine-Disrupting Chemicals, Triclosan and Octylphenol, via an Estrogen Receptor-Dependent Signaling Pathway in Cellular and Mouse Xenograft Models, scientists performed both in vitro tests on human breast cancer cells in petri dishes, and in vivo tests via tissue grafts on mice. “Although the doses of EDCs were somewhat high, we did this to simulate their effects of daily exposure, as well as body accumulation due to long-term exposure, simultaneously in animal experiments,” said Kyung-Chul Choi, PhD, co-author of the research. Results of the study established that both triclosan and octylphenol interfered with the genes involved in breast cancer growth. In human […]

(Beyond Pesticides, March 13, 2014) Part deux of a 2012 study finding that sperm counts in French men had decreased 30% over the past 16 years came to a second startling conclusion in a 2014 analysis: the cause for those dramatic decreases may be pesticides. 2012 Sperm-Count Study Published in the scientific journal Human Reproduction, the landmark 2012 study showed an alarming 30 percent decrease in sperm counts across France between 1989 and 2005. Because the data for the 2012 study were drawn from Fivnat ””a French assisted reproduction technology database”” researches made sure to limit analysis to 26,600 sperm samples from otherwise virile 35-year-old men whose partners’ fallopian tubes were either blocked or missing. This control was added to ensure that the each couple’s infertility was due to these latter problems and not a problem with the man’s sperm. Broken down, the 2012 studies identified a 1.9 percent continued annual dip in sperm concentration and also found that there was a significant 33.4% decrease in the percentage of normally formed sperm over the entire 16-year period. At the time of release, the 2012 study’s authors wrote: “To our knowledge, it is the first study concluding a severe and general […]

(Beyond Pesticides, February 5, 2014) According to a new study published in Environmental Health Perspectives, chemical contaminants in waterways that mimic estrogen -endocrine disruptors- target developing heart valves in fish and impair the growth of fish hearts. The study illustrates that these hormone-mimicking compounds, which include some pesticides, pharmaceuticals, and other household chemicals often found in sewage effluent and runoff that flows into waterways, are being linked to mounting science that show serious human and environmental adverse effects. Researchers from the Fish Health Branch of the U.S. Geological Survey (USGS) and the Carnegie Institution for Science exposed zebrafish embryos to water from 19 sites in the Susquehanna, Delaware, Allegheny and Shenandoah watersheds. Water from 16 of the sites triggered proteins in the fish that were estrogen receptors, indicating that the rivers contained endocrine disrupting chemicals. These receptors are attached to DNA, which turn genes on and off. While such activity is common in the liver, this is the first experiment to show estrogenic activity in heart valves. “This tells us that endocrine-disrupting chemicals could lead to improper heart development. We were quite surprised, since this is something that others hadn’t observed before,” said study co-author Luke Iwanowicz, PhD, and research […]

(Beyond Pesticides, November 6, 2013) A federal district court approved a settlement Monday requiring the U.S. Fish and Wildlife Service to better protect California red-legged frogs from seven common pesticides known to be highly toxic to amphibians. The settlement gives the agency two years to prepare “biological opinions” under the Endangered Species Act (ESA) to analyze pesticide use in and near the frog’s aquatic and upland habitats.   A 2006 legal settlement secured by the Center for Biological Diversity (CBD) required the U.S. Environmental Protection Agency (EPA) to assess pesticide impacts on red-legged frogs and to then formally consult with the Fish and Wildlife Service (FWS) under the ESA. The EPA’s assessments found that widespread use of pesticides is likely harming red-legged frogs and the court ordered temporary pesticide use restrictions that remain in effect today. EPA determined that 64 other pesticides are “likely to adversely affect” or “may affect” red-legged frogs. Despite the EPA’s findings, however, FWS and EPA failed to complete the required consultation, resulting in the litigation by CBD that culminated in Monday’s settlement. The court order gives FWS two years to complete biological opinions for seven pesticides: glyphosate, malathion, simazine, pendimethalin, permethrin, methomyl and myclobutanil. This […]

(Beyond Pesticides, October 28, 2013) Scientists at Washington State University (WSU), in a laboratory study,  determined that exposure to the insecticide DDT ””banned in the U.S. since 1972, but still used today in developing countries for malaria abatement programs””impacts multiple generations, ultimately contributing to obesity three generations down the line. The study, published in the journal BMC Medicine, provides the scientific community with new information on multi-generational impacts of pesticide exposure. Lead researcher Michael Skinner, PhD., professor of biological sciences at WSU, and colleagues exposed pregnant rats to DDT to determine the long-term impacts to health across generations. The study, Ancestral dichlorodiphenyltrichloroethane (DDT) exposure promotes epigenetic transgenerational inheritance of obesity, finds that the first generation of rats’ offspring developed severe health problems, ranging from kidney disease, prostate disease, and ovary disease, to tumor development. Interestingly, by the third generation more than half of the rats have increased levels of weight gain and fat storage. In other words, the great grandchildren of the exposed rats are much more likely to be obese. “Therefore, your ancestors’ environmental exposures may influence your disease development even though you have never had a direct exposure,” the study finds. Previous studies have demonstrated that exposure to […]

(Beyond Pesticides, June 28, 2013) The U.S. Environmental Protection Agency (EPA) released a draft “State of the Science” report last week acknowledging that low dose responses “do occur in biological systems” while defending its current risk assessment procedures as adequate for evaluating low dose effects. This report comes after EPA’s long running failure to fully implement a 1996 Congressionally mandated program to evaluate endocrine disruptors, and heavy criticism last year from prominent scientists who said EPA’s testing procedures are outdated. In 1999 the Natural Resources Defense Council (NRDC) filed a lawsuit against the EPA for failing to meet a statutory deadline for implementation of the Endocrine Disruptor Screening Program (EDSP) required under the 1996 Food Quality Protection Act, forcing the EPA to make a settlement agreement. As a result of  NRDC et al. v. EPA (No. C-99-03701 CAL) filed in the Northern District of California, EPA agreed to start requiring screening and testing of certain chemicals varying by date, using a tiered system. EPA’s two-tiered screening and testing system, requires that EPA will identify which chemicals are able to interact with the endocrine system in Tier 1. Tier 2 screening process was designed to go one step further, requiring EPA […]

(Beyond Pesticides, June 19, 2013) The Director of the National Institute of Environmental Health Sciences (NIEHS), Linda Birnbaum, PhD, is being criticized by some Republicans for authoring an article that describes linkages between endocrine disrupting chemicals and the onset of disease, as well as the need to understand and monitor the effects of these chemicals. Instead of encouraging efforts for greater understanding of these chemicals, the members of Congress instead blasted the article as a potential breach of National Institutes of Health (NIH) policy. NIEHS, a program of NIH, seeks to reduce the burden of human illness and disability by understanding how the environment influences the development and progression of human disease. The short article entitled, “When environmental chemicals act like uncontrolled medicine,” published online on May 7, 2013, in Trends in Endocrinology and Metabolism, lays out the case that environmental chemicals can produce unwanted endocrine effects, leading to an increase in certain diseases.  It states, “In the same way as physicians endeavor to understand and monitor the effect of medicines on endocrine pathways, we ought to achieve the same understanding and control of the effects on environmental chemicals.” Dr. Birnbaum also notes, “The proliferation of inadequately tested chemicals in […]

(Beyond Pesticides, March 1, 2013) In a literature review published in Toxicology last week, researchers found that environmental and occupational pesticide exposure was strongly associated with declines in sperm count. Researchers Sheena Martenies, BS, and Melissa Perry, ScD., MHS., determined that of the 17 studies evaluated, 15 of them reported significant associations between pesticides and semen quality. The researchers counted semen quality according to concentration of sperm over an area, their motility and ability to move, as well as their shapes. Researchers targeted studies on DDT, HCH, and abamectin, grouping pyrethroids and organophosphates by class. What they found was striking: almost all the studies reported a decrease in sperm concentration; decreased motility was also reported though less frequently; while morphological changes were not strongly associated in studies””only two indicated any changes to sperm shape. These findings build on a growing body of evidence that pesticide exposure at environmental or occupational levels diminished sperm health. In addition to the U.S. findings,  studies conducted on French, New Zealander, Indian, Tunisian, and Israeli men have all found decline in sperm count. Some studies record a drop by approximately 50% between 1940 and 1990, no small amount. These results might not be surprising as […]

(Beyond Pesticides, February 20, 2013) A new report by the United Nations Environment Programme (UNEP) and the World Health Organization (WHO) has identified endocrine disrupting chemicals as having significant health implications for the global population. According to the report, these chemicals have the capacity to interfere with tissue and organ development and function, and therefore they may alter susceptibility to different types of diseases throughout life, and represents a global threat that needs to be resolved. The report cites insufficient reporting and information on chemicals in products, materials and goods and calls for more research and collaboration. The State of the Science of Endocrine Disrupting Chemicals,   a joint study by UNEP and WHO, finds that endocrine disrupting chemicals (EDCs) are important environmental risk factors for endocrine diseases. Exposures during critical phases of development play an important role in the onset of many diseases, affecting future generations. Trends indicate an increasing burden of certain endocrine diseases across the globe, and it is clear from human studies that populations are exposed to perhaps hundreds of environmental chemicals at any one time. This UN study, which is the most comprehensive report on EDCs to date, highlights some association between exposure to EDCs […]

(Beyond Pesticides, October 2, 2012) The herbicide atrazine may be linked to an amplified risk of choanal atresia, a congenital abnormality of the nasal cavity, according to researchers at Baylor College of Medicine and other Texas institutions. Choanal atresia is recognized when tissue formed during fetal development blocks an infant’s nasal cavity. Though it is a rare condition, it is considered quite serious because it can affect an infant’s ability to breathe. The study, scheduled for publication in The Journal of Pediatrics, focused on atrazine because, although there are very few risk factors for choanal atresia, endocrine disrupting chemicals are suspected to be associated with the condition. “Endocrine disrupters aren’t fully understood, but it is believed they interfere with or mimic certain hormones, thereby blocking their proper function and potentially leading to adverse outcomes,” said Dr. Phillip Lupo, lead author of the study. Looking at mothers from Texas counties with the highest levels of estimated atrazine application, researchers discovered that they are 80 percent more likely to have children with choanal atresia or stenosis (a less severe form of the condition) than compared to mothers who live in counties with the lowest levels. The herbicide atrazine and over 50 other […]

(Beyond Pesticides, June 21st, 2012) A new study details the toxic effects of long-term exposure to commonly used agricultural pesticides. Results indicate an increased likelihood of moderate to severe blood toxicity and a reduced total number of bone marrow cells, which can lead to degenerative diseases like aplastic anemia. The study, entitled “Pesticide Induced Alterations in Marrow Physiology and Depletion of Stem and Stromal Progenitor Population: An Experimental Model to Study the Toxic Effects of Pesticide” is published in the online version of the Journal of Environmental Toxicology . The experiment, led by researchers at the Calcutta School of Tropical Medicine’s Department of Biochemistry and Medical Biotechnology, exposed a group of mice to a mixture of organochlorine, organophosphate and pyrethriod pesticides, including a preponderance of the chemicals cypermethrin, and chloropyrofos. The exposed mice showed an overall reduction in the ability of their bodies to produce bone marrow cells. Bone marrow, the soft flexible tissue found in the interior of bones, is a storehouse for stem cells. While the exact mechanism is unknown to researchers, the study reveals that the microenvironment in which stem cells develop is somehow deranged by pesticides. This derangement prevents the maturation of stem cells into every […]

(Beyond Pesticides, June 18, 2012) Once again, attempts to repeal the Clean Water Act permits for pesticide discharges are underway in Congress. This week the 2012 Farm Bill will be introduced to the Senate floor where measures to attack environmental laws have been added unceremoniously to the bill, including the controversial H.R. 872, Reducing Regulatory Burdens Act, which seeks to undermine federal authority to monitor our nation’s waterways for pesticide contamination, as well as eroding already lax federal oversight of genetically engineered crops. Congress is working on the monumental process of altering and renewing the Farm Bill, S. 3240. This process only comes along every five years and has major impacts on how we grow our food. The last Farm Bill was passed in 2008, and expires in 2012. The bill is expected to be introduced on Senate floor on Tuesday, June 19. North Carolina Democratic Senator Kay Hagan and Idaho Republican Senator Mike Crapo have introduced an amendment (amendment 2367, full text available in the Congressional Record) to the Farm Bill that states pesticides should be allowed into water bodies without any oversight, leaving the public to swim, fish and boat on waters that are contaminated with endocrine disruptors, […]

(Beyond Pesticides, May 22, 2012) The Spanish-based Inesfly company announced recently its plans to release commercially pesticide encapsulated paint, Inesfly 5A IGR, containing two neurotoxic organophosphates (OPs), chlorpyrifos and diazinon, and the insect growth regulator (IGR), pyriproxyfen, which it hopes will combat malaria in sub-Saharan Africa. The company’s owner Pilar Mateo, PhD, calls her product “a vaccine for houses and buildings” and explains that because the insecticides are released slowly from the paint, it remains effective for two to four years. This formulation of Dr. Mateo’s paint could not be registered for use in the U.S. because both indoor residential uses of chlorpyrifos and diazinon have been banned because of risks posed to children’s health, although the company has another formulation that substitutes pyrethroids for the organophosphates. Though probably well-intentioned —Dr. Mateo has already invested $6 million of her family’s money and $12 million in grants from nonprofits, on research, creating educational programs about hygiene, and donating paint to more than 8,000 homes in Latin America and Africa””the product puts the people it is supposed to protect from disease at risk for other health problems. Organophosphate insecticides have been linked to a host of neurodevelopmental problems, especially in children. Because […]

(Beyond Pesticides, March 16, 2012) A report published online this week in the journal Endocrine Reviews documents extensive scientific research showing that endocrine disrupting chemicals, or endocrine disruptors, can be toxic to humans even in minutely small doses. The report, three years in the making, was published Wednesday by a team of 12 scientists who study hormone-altering chemicals. Authors include the University of Missouri’s Frederick vom Saal, PhD., who has linked low doses of bisphenol A (BPA) to a variety of effects, Theo Colborn, PhD., who is credited with first spreading the word about hormone-disrupting chemicals in the late 1980s, and University of California at Berkeley’s Tyrone Hayes, PhD., who has documented the effects of the pesticide atrazine on frogs. Drs. Colborn, Hayes, and vom Saal are all former speakers at the National Pesticide Forum. One of the reporrt’s authors is Pete Myers, PhD, the founder of Environmental Health News and chief scientist of Environmental Health Sciences. Dozens of substances that can mimic or block estrogen, testosterone and other hormones are found in the environment, the food supply and consumer products, including plastics, pesticides and cosmetics. One of the biggest, longest-lasting controversies about these chemicals is whether the tiny doses […]

(Beyond Pesticides, February 13, 2012) Acclaimed ecologist and Living Downstream author, Sandra Steingraber, will be speaking at the 30th National Pesticide Forum. With Connecticut and communities throughout the country facing threats to existing environmental laws, as well as opportunities for greater protection and increased local control, this conference will have a strong focus on organic land management and protective policies. Join Dr. Steingraber and other researchers, authors, beekeepers, organic business leaders, elected officials, activists, and others to discuss the latest science, policy solutions, and grassroots action. Registration Register online. Fees start at $35 ($15 for students) and include all sessions, conference materials, and organic food and drink. Speaker Highlights Sandra Steingraber, PhD – An acclaimed ecologist and author, Dr. Steingraber explores the links between human rights and the environment, with a focus on chemical contamination. She takes a personal and scientific look at these issues and offers insights into how we can protect our environment and ourselves. She brings a clear, lyrical voice to the complex evidence of biology. The author of several books, including her latest, Raising Elijah, Dr. Steingraber has been called “a poet with a knife” by Sojourner magazine, and received many honors for her work as […]

(Beyond Pesticides, January 31, 2012) The Environmental Protection Agency (EPA) has proposed an expansion in pyrethrins/pyrethroid insecticide uses as part of its cumulative risk assessment for this neurotoxic class of chemicals. In the cumulative risk assessment, EPA concludes that pyrethroids “do not pose risk concerns for children or adults,” ignoring a wealth of independent data that links this class of chemicals to certain cancers, respiratory and reproductive problems, and the onset of insect resistance. It went as far as to state that its cumulative assessment supports consideration of registering additional new uses of these pesticides, potentially opening the flood gates for manufacturers to bombard the market with more pyrethroid pesticides, endangering the health of the public. The agency is accepting public comments through February 8, 2012. Tell EPA that it has ignored numerous health effects and that these pesticides do pose unacceptable risks to human health given the availability of alternatives. Submit comments directly to the EPA docket or sign-on to Beyond Pesticides’ comments. In its comments to EPA, Beyond Pesticides states: There are several major concerns and flaws plaguing this cumulative assessment, which therefore does not meet the regulatory burden in fully evaluating synthetic pyrethroids’ effect on public and […]

(Beyond Pesticides, December 15, 2011) New research suggests that the recent re-emergence of bed bug infestations may originate from insecticide use in the tropics. According to the results, which were presented at the American Society of Tropical Medicine and Hygiene’s 60th annual meeting, exposure to treated bed nets and linens caused populations of bed-bugs to build resistance to those chemicals. The findings presented at the gathering showed that 90% of 66 populations sampled from 21 U.S. states were resistant to a group of insecticides, known as pyrethroids, commonly used to kill unwanted bugs and flies. Other research has already shown that an over-reliance on chemical controls over the years has helped bed bugs evolve to be resistant to these chemicals. One of the co-authors, evolutionary biologist Warren Booth, Ph.D. from North Caroline State University in Raleigh, told the BBC news that the genetic evidence he and his colleagues had collected show that the bed-bugs infecting households in the U.S. and Canada in the last decade are not domestic bed bugs, but imports. The team collected samples from across the eastern U.S. and discovered populations of bed-bugs that are genetically very diverse. “If bed-bugs emerged from local refugia, such as poultry […]

(Beyond Pesticides, October 20, 2011) The Center for Biological Diversity filed a lawsuit yesterday against the Environmental Protection Agency (EPA) and U.S. Fish and Wildlife Service (FWS) for failing to evaluate and act on threats to the threatened California red-legged frog posed by more than 60 toxic pesticides used in and near its habitats. The Center had announced its intent to sue the two agencies back in December 2010. A 2006 legal settlement secured by the Center requires EPA to assess the impacts of harmful pesticides on red-legged frogs and formally consult with the Fish and Wildlife Service to address those impacts. EPA determined that widespread use of more than 60 pesticides is likely harming red-legged frogs, but since the agency and the Fish and Wildlife Service have failed to complete the required evaluations, no permanent protections for frogs have been put in place. “Federal agencies acknowledge that scores of pesticides may harm California’s rare red-legged frogs, but for years now they’ve neglected to complete biological evaluations of the effects of these chemicals,” said Jeff Miller, a conservation advocate with the Center. “California’s imperiled frogs are suffering as a result.” “Biological opinions,” the evaluations required by the Endangered Species Act, […]

(Beyond Pesticides, September 26, 2011) On September 23, 2011, the Centers for Disease Control and Prevention (CDC) published a study in its Morbidity and Mortality Weekly Report linking pesticides sprayed in attempts to control bed bugs to poisoning incidents and death. Because bed bugs do not transmit disease and can be controlled without pesticides, this risk is completely unnecessary. The study, “Acute Illnesses Associated with Insecticides Used to Control Bed Bugs,” utilized data from California, Florida, Michigan, North Carolina, New York, Texas, and Washington. In those seven states, over 100 poisonings, including one fatality, were associated with bed bug-related insecticide use. The CDC researchers used data from states participating in the Sentinel Event Notification System for Occupational Risks (SENSOR)-Pesticides program and the New York City Department of Health and Mental Hygiene (NYC DOHMH). The authors defined “acute illness” associated with an insecticide used to control bed bugs as two or more acute adverse health effects resulting from exposure to an insecticide used for bed bug control. The study reports: A total of 111 illnesses associated with bed bug–related insecticide use were identified; although 90 (81%) were low severity, one fatality occurred. Pyrethroids, pyrethrins, or both were implicated in 99 (89%) […]

(Beyond Pesticides, August 11, 2011) A combination of eleven different kinds of commonly used pyrethroids were tested on mice in a new study which found that, at real-world exposure levels, the insecticides can produce heightened toxicity that is equal to the sum of each insecticide’s individual effect. The mixture of similar-acting insecticides works by over-stimulating electronic channels in the mouse’s brain cells and eventually causing death. This study adds to the growing body of research on the toxicity of pesticide combinations in nature and showcases the need for policy change because the current risk assessment approach to regulating pesticides fails to look at chemical mixtures and synergistic effects. The U.S. Environmental Protection Agency (EPA) currently regulates on a chemical-by-chemical basis, but as this study demonstrates, interacting chemicals can have synergistic effects at very low levels, where a “chemical cocktail” of multiple interacting chemicals combine to have greater effects than expected. Pesticides can also have a cumulative “toxic loading” effect both in the immediate and long term. Researchers exposed mice brain cells to eleven different food-use pyrethroid insecticides either singly or in a mixture in the study entitled ”Additivity of pyrethroid actions on sodium influx in cerebrocortical neurons in primary culture.” […]

(Beyond Pesticides, July 22, 2011) Parallel bills have been introduced in the U.S. Senate and U.S. House of Representatives designed to increase federal research on endocrine disrupting chemicals and ensure public safety by restricting or eliminating chemicals found to present unacceptable risks to public health. S 1361, introduced by Senator John Kerry (D-MA), and HR 2521, introduced by Rep. Jim Moran (D-VA), are both titled the Endocrine-Disrupting Chemicals Exposure Elimination Act. The bills would establish a scientific panel at the National Institute of Environmental Health Sciences (NIEHS) to evaluate up to ten chemicals per year that potentially affect the endocrine system and would create a trigger to ban those found most harmful to public health. The bills would create a more updated scientific evaluation process than any that currently exists in the federal government for reviewing potential endocrine disruptors and would have a strong regulatory mandate to ban or restrict chemicals that are found to present serious health risks. The specific process outlined directs the National Toxicology Program at NIEHS to evaluate each chemical according to (i) the amount of evidence that it is an endocrine disruptor, (ii) the “level of concern” that it may disrupt hormones, and (iii) the […]

(Beyond Pesticides, July 22, 2011) Parallel bills have been introduced in the U.S. Senate and U.S. House of Representatives designed to increase federal research on endocrine disrupting chemicals and ensure public safety by restricting or eliminating chemicals found to present unacceptable risks to public health. S. 1361, introduced by Senator John Kerry (D-MA), and H.R. 2521, introduced by Rep. Jim Moran (D-VA), are both titled the Endocrine-Disrupting Chemicals Exposure Elimination Act. The bills would establish a scientific panel at the National Institute of Environmental Health Sciences (NIEHS) to evaluate up to ten chemicals per year that potentially affect the endocrine system and would create a trigger to ban those found most harmful to public health. The bills would create a more updated scientific evaluation process than any that currently exists in the federal government for reviewing potential endocrine disruptors and would have a strong regulatory mandate to ban or restrict chemicals that are found to present serious health risks. The specific process outlined directs the National Toxicology Program at NIEHS to evaluate each chemical according to (i) the amount of evidence that it is an endocrine disruptor, (ii) the “level of concern” that it may disrupt hormones, and (iii) the […]

(Beyond Pesticides, May 9, 2011) The Endocrine Disruptor Exchange Inc. (TEDX), founded by Theo Colborn, PhD, has released a list of chemicals with the potential to affect the endocrine system. According to TEDX, every chemical on the TEDX List has one or more verified citations to published, accessible, primary scientific research demonstrating effects on the endocrine system. Endocrine disruptors are chemicals that impact traditional endocrine glands, their hormones and receptors such as estrogens, anti-androgens, and thyroid hormones. To date there are approximately 800 endocrine disruptors on the TEDX List. Download the TEDX List (Excel) Many everyday chemicals that people are exposed to can wreak havoc on the body’s endocrine system. Pesticides such as triclosan, atrazine, permethrin and many others have been associated with effects on the body’s hormone system. Visit the Pesticide Induced Disease Database for more on the chemicals linked to endocrine disruption. Endocrine effects include direct effects on traditional endocrine glands, their hormones and receptors such as estrogens, anti-androgens, and thyroid hormones, as well as signaling cascades that affect many of the body’s systems, including reproductive function and fetal development, the nervous system and behavior, the immune and metabolic systems, the liver, bones and many other organs, glands […]