(Beyond Pesticides, July 23, 2009) A new study published in the August 2009 issue of Environmental Toxicology and Chemistry found that insecticides used in highly populated agricultural areas of California’s Central Valley affect amphibians that breed in the Sierra Nevada Mountains to the east. This study adds to the increasing evidence that pesticides impact areas and wildlife species that are miles from sources of pesticide application. Researchers from the Cooperative Wildlife Research Laboratory, Southern Illinois University and U.S. Geological Survey (USGS) examined the chronic toxicity of two of the insecticides most commonly used in the Central Valley- chlorpyrifos and endosulfan, to larval Pacific treefrogs (Pseudacris regilla) and foothill yellow-legged frogs (Rana boylii), the amphibians with declining populations that live and breed in meadows surrounding the Sierra Nevada. The results are discussed in “Toxicity of Two Insecticides to California, USA, Anurans and Its Relevance to Declining Amphibian Populations.” The study used laboratory testing to examine how the insecticides affected the two frogs at environmentally realistic concentrations. During testing, tadpoles were observed at various stages of development to see how the insecticides affected their growth and health. The researchers found that endosulfan was more toxic than chlorpyrifos to both species, and tadpoles […]

(Beyond Pesticides, July 6, 2009) Last week, the U.S. Environmental Protection Agency (EPA) proposed to formally evaluate the harmful effects of 74 pesticides on 11 endangered and threatened species in the San Francisco Bay Area over the next five years, and to impose interim restrictions on use of these pesticides in and adjacent to endangered species habitats. The proposal stems from a settlement agreement with the Center for Biological Diversity, which sued EPA in 2007 for violating the Endangered Species Act by registering and allowing the use of toxic pesticides in Bay Area endangered species habitats without determining whether the chemicals jeopardize those species’ existence. “Tens of millions of pounds of toxic and poisonous chemicals, known to be deadly to endangered species and harmful to human health, including proven carcinogens and endocrine disruptors, are applied in the Bay Area each year, and many of those find their way through runoff or drift into our soil, creeks and rivers, San Francisco Bay, and sensitive wildlife habitats,” said Jeff Miller, conservation advocate with the Center. “The toxic stew of pesticides in the Bay-Delta has played a major role in the collapse of native fish populations, and pesticides are a leading cause of […]

(Beyond Pesticides, April 29, 2009) The U.S. Environmental Protection Agency (EPA) announced earlier this month that it is finally ready to move forward with preliminary testing of 67 active and inert pesticide ingredients for possible endocrine disrupting effects. But, according to prominent researcher and author Theo Colborn, PhD, these tests are outdated, insensitive, crude, and narrowly limited, and will fail to detect many serious effects on human development. In an eye-opening opinion-editorial published in Environmental Health News, Dr. Colborn, founder and president of The Endocrine Disruption Exchange, and Co-author of Stolen Future, says that EPA’s testing protocol is “a pitiful skeleton” of what it needs to be. The tests to be used by EPA were first recommended in 1998. Since then the science has made progress and become more sophisticated. Current research is based on different assumptions than the toxicological assumptions that first drove the EPA test designs. However, EPA has not updated its protocol. Each of EPA’s tests and assays was designed under the surveillance of corporate lawyers who had bottom lines to protect and assorted toxicologists who were not trained in endocrinology and developmental biology. For over a decade, EPA ignored the vast wealth of information on endocrine […]

(Beyond Pesticides, January 22, 2009) A new English study has found that chemicals found in rivers and waste waters could be linked to male infertility. These chemicals, known as anti-androgens, block the action of the male sex-hormone testosterone and could impact the development of male reproductive organs in humans. The study entitled, “Statistical Modeling Suggests That Anti-Androgens in Wastewater Treatment Works Effluents Are Contributing Causes of Widespread Sexual Disruption in Fish Living in English Rivers,” and published in the journal Environmental Health and Perspectives identifies a group of river pollutants that cause testosterone to stop working. These chemicals, released from wastewater treatment operations into rivers and other surface waters are responsible for the feminization of male fish and abnormalities in male reproductive organs. Previous studies have attributed these problems in male fish to estrogenic compounds also found in surface waters. However, this study is the first to identify anti-androgens with the feminization of male fish. It also indicates that male feminization may be the result of a rather more complicated interaction taking place between different classes of pollutants, resulting in an endocrine disrupting chemical cocktail. “We have identified a new group of chemicals in our study on fish, but we […]

(Beyond Pesticides, January 13, 2009) In another stinging defeat for the Bush Environmental Protection Agency (EPA), on January 7, 2009, the 6th Circuit Court of Appeals issued a clear rebuke of the administration’s 2006 rule which exempted certain commercial pesticide applications from the oversight provided by Congress under the Clean Water Act. [The National Cotton Council et al. v. EPA (Nos. 06-4630; 07-3180/3181/3182/3183/3184/3185/3186/3187/3191/3236). See also Headwaters, Inc. v. Talent Irrigation Dist., 243 F.3d 526, 532-33 (9th Cir. 2001).] The Court held that pesticide residuals and biological pesticides constitute pollutants under federal law and therefore must be regulated under the Clean Water Act (CWA) in order to minimize the impact to human health and the environment. According to Beyond Pesticides, the EPA rule had allowed the weaker and more generalized standards under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA) to trump the more stringent CWA standards. CWA uses a kind of health-based standard known as maximum contamination levels to protect waterways and requires permits when chemicals are directly deposited into rivers, lakes and streams, while FIFRA uses a highly subjective risk assessment with no attention to the safest alternative. Read Beyond Pesticides’ press release on EPA’s 2006 decision. Several manufacturers […]

(Beyond Pesticides, January 9, 2009) Environmental Toxicology and Chemistry has published a study (Vol. 27(12):2496—2500) entitled “Effects of Malathion on Embryonic Development and Latent Susceptibility to Trematode Parasites in Ranid Tadpoles.” It shows that malathion used as an agricultural insecticide is responsible for interfering with the normal development of pickerel frog embryos, thus leaving them more susceptible to parasite invasion. Malathion is present in natural water sources that have been exposed to urban and agricultural runoff. This organophosphate pesticide can be applied by planes in mosquito control program, and as esult enters water from the air. Although direct lethal and sublethal effects of chemical contaminants have been documented, latent and long-term effects have been less well documented. Therefore, researchers sought to fill this knowledge gap and found, as suspected, that tadpole survival rates decreased and malformations and susceptibility to parasite encystment rates increased as a result of exposure to malathion concentrations mimicking those found in actual water sources. Tadpoles are being exposed to increasing numbers of parasites in waters that are warming as a result of global climate change, and the researchers who performed this study speculate that, as a consequence, those exposed to malathion will have weakened immune systems […]

(Beyond Pesticides, December 18, 2008) The New Zealand’s Environmental Risk Management Authority (ERMA) has announced it will ban the controversial organochlorine pesticide endosulfan, effective January 16, 2009. Endosulfan, already banned in numerous countries including all the European Union countries, is an insecticide used on a wide range of fruits and vegetables and also on athletic fields in New Zealand. Illegal residues have been found in beef destined for South Korea, resulting in enormous costs for New Zealand exporters. Use of endosulfan for agriculture continues in the U.S., despite causing severe health and environmental problems. A coalition of groups, including the Pesticide Action Network Aotearoa New Zealand (PAN ANZ), Soil and Health Association and Safe Food Campaign, have long campaigned for the banning of endosulfan. Earlier this year, the three organizations carried out a number of residue tests on produce to draw attention to the extent of endosulfan residues, especially in tomatoes. “We are delighted that ERMA has overturned its earlier ”˜proposed’ decision to keep using this pesticide,” stated Meriel Watts, Ph.D., co-coordinator of PAN ANZ. “It would have been deeply embarrassing for New Zealand to continue its use when the pesticide has entered the process for a global ban under […]

(Beyond Pesticides, November 17, 2008) A toxic soup of the most commonly used pesticides frequently detected in nature can adversely affect the environment and decimate amphibian populations even if the concentration of the individual chemicals are within limits considered safe, according to University of Pittsburgh research published in the online edition of Oecologia. The results of this study build on a nine-year effort to understand potential links between the global decline in amphibians, routine pesticide use, and the possible threat to humans in the future. Amphibians are considered an environmental indicator species because of their unique sensitivity to pollutants. Their demise from pesticide exposure could foreshadow the fate of less sensitive animals, according to study author Dr. Rick Relyea, Ph.D., an associate professor of biological sciences in the University of Pittsburgh’s School of Arts and Sciences. Leopard frogs, in particular, are vulnerable to contamination; once plentiful across North America, their population has declined in recent years as pollution and deforestation has increased. Dr. Relyea exposed gray tree frog and leopard frog tadpoles to small amounts of the ten pesticides that are widely used throughout the world. Dr. Relyea selected five insecticides: carbaryl, chlorpyrifos, diazinon, endosulfan, and malathion; and five herbicides: […]

(Beyond Pesticides, October 27, 2008) Six pesticides that threaten water quality and public health continue to be detected in groundwater samples, according to a new U.S. Geological Survey (USGS) study. Published in the Journal of Environmental Quality, the study evaluates groundwater contaminants from samples taken from over 300 wells across the U.S. Over the years, frequent research has detected pesticides in ground water around the country, including in aquifers used for drinking-water supply. Over the past few decades, the use of some pesticides has been restricted or banned, while new pesticides have been introduced. One goal of the study was to track the retention of various types of contaminants that would be found in the different pesticides used over the years. Original samples were taken from the wells from 1993-1995, and compared with samples taken from 2001-2003. Laboratory analysis was performed using methods that allowed detection of pesticide compounds at concentrations as small as 1,000 times below U.S. EPA drinking-water standards. Of the 80 compounds studied, six were detected in ground water from at least 10 wells during both of those sample periods. The six pesticide compounds detected are the triazine herbicides atrazine, simazine, and prometon; the acetanilide herbicide metolachlor; […]

(Beyond Pesticides, October 6, 2008) University of Pittsburgh researchers have found that the commonly used insecticide malathion can decimate tadpole populations by altering their food chain. The study, published in the October 1 edition of Ecological Applications, finds that gradual amounts of malathion that were too small to directly kill developing leopard frog tadpoles instead sparked a biological chain of events that deprived them of their primary food source. As a result, nearly half the tadpoles in the experiment did not reach maturity and would have died in nature. The results build on a nine-year effort to investigate whether there is a link between pesticides and the global decline in amphibians, which are considered an environmental indicator species because of their sensitivity to pollutants. According to the researchers, their deaths may foreshadow the poisoning of other less environmentally-sensitivespecies, including humans. According to the U.S. Environmental Protection Agency (EPA), malathion is the most commonly used insecticide in U.S. agriculture and the third most commonly used insecticide in the U.S. home and garden sector. It has been detected in the wetlands where frogs and other amphibians live. The researchers created simulated ponds from 300-gallon outdoor tanks containing wood frog and leopard frog […]

(Beyond Pesticides, September 23, 2008) According to the Minnesota Department of Agriculture’s 2007 Water Quality Monitoring Report, released in August 2008, the endocrine disrupting herbicide atrazine is detected in pristine lakes in northern Minnesota far from the agricultural fields where it is applied. Metolachlor, acetochlor and dimethenamid are also frequent contaminants, according to the statewide sampling. The report, which uses data collected by a collaborative program between the U.S. Environmental Protection Agency, the Minnesota Department of Agriculture and the Minnesota Pollution Control Agency, analyzed samples from 55 of the state’s lakes. Atrazine was detected in approximately 87% of the 2007 samples, an increase from 2006. The presence of atrazine in such a large percentage of the lakes, many of which are located in non-agricultural areas of northern Minnesota, suggests widespread atmospheric deposition of this chemical (movement through wind and rain). “To some people, it is a bit of a surprise, but the concentrations are low, very low,” Steven Heiskary, a research scientist with the Minnesota Pollution Control Agency (MPCA) told the Star Tribune. Unfortunately, this is not very reassuring, given the fact that many of the developmental impacts linked to atrazine are seen at very low levels, sometimes at just […]

(Beyond Pesticides, July 17, 2008) In a new study published in Environmental Health Perspectives, researchers have found an increased occurrence of intersex toads in areas with greater agricultural land cover. This points to a link between certain pesticides and endocrine disruption, a change in the hormonal balance that can have sexual and reproductive effects. Such results implicate pesticides in the decline of amphibian populations, and suggests that these chemicals are also adversely affecting humans. The study, which was conducted in south Florida, looked at cane toads, Bufo marinus, at five sites with differing land use patterns ranging from suburban to nearly completely agricultural (primarily sugarcane and vegetables). Researchers examined physical characteristics such as coloration, sexual organs, and forelimb length, as well as hormone concentrations, and found a higher rate of feminization for toads in agricultural areas. In these areas, glyphosate (the active ingredient in Round Up herbicide) and atrazine (an herbicide) use is common. Tyrone Hayes, PhD, was one of the first to document the endocrine disrupting effects of atrazine on frogs in a laboratory setting. Countering any doubts of why this work is important, Dr. Hayes said, “People often say, ”˜It is just frogs, so who cares?’ Well it […]

(Beyond Pesticides, June 20, 2008) University of Massachusetts (UMass) researchers have identified certain plants that can absorb excess pesticides from soil and prevent their runoff into adjacent waterways. Golf courses typically use considerable amounts of herbicides and fungicides to maintain perfectly manicured greens, much of which ends up polluting water and harming aquatic organisms. This study found that plants like blue flag iris can act as “living filters” on the edge of greens.“ Studies from golf greens have shown that five percent to ten percent of the total pesticides applied are lost in runoff. In worst case conditions, this figure can be as high as 30 percent,” says John Clark, Ph.D., a professor of veterinary and animal science and a principal investigator on the grant. “We have identified plant species that can reduce the amount of certain pesticides in soil by up to 94 percent in the greenhouse.” Blue flag iris reduced chlorpyrifos by 76 percent and levels of chlorothalonil by 94 percent after three months of growth. The study was funded by the UMass Amherst Environmental Institute, the Massachusetts Pesticide Analysis Laboratory, and the U.S. Golf Association. Interest in “greener” turf management practices have risen lately along with golf’s […]

(Beyond Pesticides, April 10, 2008) Common frogs that live in suburban areas are more likely than their rural counterparts to develop reproductive abnormalities, according to David Skelly, PhD, professor of ecology at the Yale School of Forestry and Environmental Studies. This phenomenon becomes a serious concern as the frog’s mating season begins, leaving researchers to wonder: will frogs be clear on their role in the annual ritual? Research by Dr. Skelly, soon to be published, focuses on the common green frog, Rana clamitans, within the Connecticut River Valley. A total of 233 frogs were collected from various ponds and landscapes with the river valley and among them 13 percent have abnormalities occurring in their reproductive organs. In urban areas, 18 percent of the collected frogs are intersex, and in suburban areas 21 percent. Frogs collected from agricultural areas have the lowest rate of reproductive problems with just 7 percent classified as intersex. According to Dr. Skelly, the more suburban the land cover, the more likely the abnormalities. “This is the first evidence that I think anyone has provided that agriculture is doing anything but pushing those rates higher,” remarked Dr. Skelly of the intersex phenomena. In an attempt to explain […]

(Beyond Pesticides, March 24, 2008) Scientists at the U.S. Geological Survey (USGS) announced that they have identified ten contaminants, including pesticides, in the Potomac River, which flows through downtown Washington, DC, that could be responsible for the alarming discovery of “intersex fish,” male fish producing eggs. The suspected chemicals include: atrazine, a common herbicide used in agriculture and on lawns that is already linked to sexual abnormalities in frogs; insecticides chlorpyrifos and endosulfan; the herbicide metolachlor; and two chemicals used to add fragrance to perfumes, soaps and other products, tonalide and galaxolide. To collect the samples, USGS scientists suspended a device intended to serve as a facsimile fish in the Potomac River near the Washington, DC’s Blue Plains sewage plant. The device had a plastic-coated tube, which simulated a fish’s permeable skin, and a layer of simulated fat. According to the Washington Post, the tests on this fake fat revealed a range of potentially worrisome pollutants. Most have been found in other streams around the U.S. “The types of things we’re finding are the types of things that are associated with everyday life,” David Alvarez, a U.S. Geological Survey research chemist who analyzed samples from the Potomac told the Washington […]

(Beyond Pesticides, March 12, 2008) BioDiversity Research Institute, a nonprofit ecological research group, released a new report that was presented March 12, 2008 to Maine’s Joint Standing Committee on Natural Resources. Entitled “Contaminant Screening in Maine Birds,” it documents over 100 harmful contaminants that were found in Maine bird eggs. Scientists collected 60 eggs, representing 23 species of birds, all of which test positive for chemical contaminants, some at levels believed to be harmful to the birds. Flame retardants (PBDEs), industrial stain and water repellants (PFCs), transformer coolants (PCBs), pesticides (OCs), and mercury are found in all 23 species of birds tested. The bird species studied live in a variety of habitats: on Maine’s ocean, salt marshes, rivers, lakes and uplands. “This is the most extensive study of its kind to date and the first time industrial stain and water repellants were discovered in Maine birds,” says report author, senior research biologist Wing Goodale with the Institute. The Common loon, Atlantic puffin, piping plover, belted kingfisher, great black-backed gull, peregrine falcon and bald eagle have the highest contaminant levels. The flame retardant deca-BDE, banned last year in Maine, is found in eight species. Overall, eagles carry the greatest contaminant load, […]

(Beyond Pesticides, February 19, 2008) Common household chemicals and widespread pollutants are changing male reproductive health and impacting sexual function, development and cancer rates of today’s generations and possibly their offspring, according to more than 15 years of research by a Colorado State University expert. For example, one study looking at sperm counts globally from 1940, when chemicals first began to be widely produced, to the 1990s, indicates a 1.15 percent per year decline in sperm counts. These declines may be linked to chemical exposure.Rao Veeramachaneni, BVSc, MScVet, PhD, a biomedical sciences professor in the College of Veterinary Medicine and Biomedical Sciences, has found that chemicals including pesticides, common pollutants in ground water, and chemicals in plastics, make-up and nail polish are on a growing list of culprits causing developmental abnormalities such as impaired sperm quality and impotence. Reproductive health can be compromised if males are exposed at various times in life spanning from in utero up to adulthood.Dr. Veeramachaneni’s findings span the globe and are across species lines, from humans to horses, wildlife to frogs. His research, coupled with the collective findings of other experts in the field, indicates a strong link related to pollutants, and incidence of such […]

(Beyond Pesticides, December 11, 2007) Arturo Rodriguez (UFW President), Devra Davis, Ph.D. (author and University of Pittsburgh professor of epidemiology) and Tyrone Hayes, Ph.D. (UC Berkeley professor of integrative biology) will be speaking at the 2008 National Pesticide Forum. The conference, Reclaiming Our Healthy Future: Political change to protect the next generation, will be held March 14-16, 2008 at the University of California, Berkeley. A native of Texas, Arturo S. Rodriguez has worked tirelessly to continue the legacy of Cesar Chavez since taking over the helm of the United Farm Workers of America (UFW) upon the death of its legendary founder in 1993. Beyond winning fair contracts for its workers, the UFW continues to work to protect farmworkers from pesticides and other workplace hazards. Recent union victories are agreements with Gallo Vineyards Inc. and Coastal Berry Co., the largest winery and the largest strawberry employer in the U.S., as well as pacts protecting winery workers in Washington and mushroom workers in Florida. Devra Davis, Ph.D., a renowned environmental health expert, is professor of epidemiology at the University of Pittsburgh Graduate School of Public Health. Dr. Davis was designated a National Book Award Finalist for her book, When Smoke Ran Like […]

(Beyond Pesticides, August 2, 2007) According to the Associated Press (AP), a new study finds frogs in Vermont living near farms are more than twice as likely as those living elsewhere to have deformities like missing legs. Yale University ecologist David Skelly, Ph.D., told the AP he decided to look at Vermont frogs because the state has been a hot spot during the last 10 years for deformed frogs. “We went to all these wetlands and cataloged where the deformities were found, and what kind of landscapes seemed to pose higher risks, if any,” Dr. Skelly said. “The answer was, frogs growing up in proximity to agriculture were more than two times as likely to have deformities. This doesn’t say it is chemical pesticides, but you can’t credibly consider this problem of the frogs without at least evaluating whether pesticides are involved.” Richard Levey, a biologist with the Vermont Agency of Natural Resources, said two common farm chemicals, atrazine and metolachlor, had been found in trace amounts in water from wetlands where deformed frogs had been found. But he told the AP that the concentrations were far below those thought to have any effect on aquatic life. Research by Tyrone […]

(Beyond Pesticides, July 9, 2007) New field and laboratory research reveals that organochlorine pesticides reduce the hatching rate of alligator eggs. This finding adds to previous studies demonstrating that pesticides cause endocrine disruption in alligators, resulting in poorly developed reproductive organs. American alligators, which were delisted from the endangered species list in the late 1980s, are still experiencing low hatching rates around contaminated wetlands. In Florida, many wetlands were converted or are adjacent to farmlands and some farmlands have been restored back into wetlands in efforts to improve ecosystem health. Agricultural runoff has altered the surrounding aquatic environments by depositing pesticide residuals and skewing nutrient levels. Organochlorine pesticides, such as chlordane, toxaphene and dieldrin, have been especially problematic due to their persistent nature. The link between pesticides and reproductive problems in alligators has been firmly established since the 1990s. University of Florida zoologist Louis Guillette, Ph.D., found pesticides are linked to smaller penises and abnormal egg development in exposed alligators. Florida Fish and Wildlife Conservation Commission official Allan Woodward has also observed fewer hatches around lakes with high pesticide levels. He reports the hatching rate has improved around Lake Apopka, a large lake in central Florida, during the last two […]

(Beyond Pesticides, July 3, 2007) A scientist at the Minnesota Pollution Control Agency (MPCA) has filed a whistleblower lawsuit in federal court, arguing that he was first not allowed to share his research with a legislative panel, and then fired for it. Hydrologist Paul Wotzka, who worked for the state Agriculture Department for sixteen years before leaving to join MPCA in October, had been slated to testify about the rise in atrazine levels in Whitewater State Park, which have exceeded recommended levels for several area species. Atrazine, which has been linked to cancer, birth defects, neurotoxicity, endocrine disruption and a variety of other health and environmental effects and has been banned by the European Union, is a widely used agricultural herbicide.. Mr. Wotzka said in his lawsuit, as well as publicly, that this rise in atrazine in Minnesota waterways was due to increased row-crop agriculture in the region and the the Agriculture Department’s support of corn for ethanol. In his lawsuit, Mr. Wotzka is asking for $75,000 and to return to his position with MPCA. He claims that he was put on investigative leave, “related to missing data that is property of the State of Minnesota.” The investigative leave led […]

(Beyond Pesticides, June 12, 2007) More than 10 years after being directed to do so by Congress, the Environmental Protection Agency (EPA) has announced that it will test 73 pesticides for their potential to damage the endocrine system and disrupt the normal functioning of hormones in the body, the agency announced in a press release yesterday. EPA is seeking comments on the draft list of 73 pesticides to be evaluated under the new screening regimen. The 1996 Food Quality Protection Act (FQPA) set a 1999 deadline for EPA to develop a battery of assays with which pesticide manufacturers will be required to screen their products as possible endocrine (hormonal) disrupters, similar to tests required to determine whether chemicals cause cancer, birth defects, genetic mutations, or other problems. EPA has repeatedly pushed back the deadline and despite claims to be “a leader in endocrine disruptor research,” EPA has yet to test a single chemical under the protocol. EPA draft list of 73 pesticide ingredients, including both active and inert ingredients, were chosen based on their relatively high potential for human exposure. According to the press release, priority was given “to pesticide active ingredients where there is the potential for human exposure […]

(Beyond Pesticides, June 4, 2007) The breakdown products (oxons) of the three most commonly used organophosphate pesticides in California’s agricultural Central Valley — chlorpyrifos, malathion and diazinon – are 10 – 100 times more toxic to amphibians than their parent compounds, which are already highly toxic to amphibians, according to a study released last Wednesday by scientists of Southern Illinois University, Carbondale, and the U.S. Geological Survey (USGS) Western Ecological Research Center. The results of the laboratory experiments on the toxicity of the three breakdown products were published in the journal Environmental Pollution. The title of the article is “Comparative toxicity of chlorpyrifos, diazinon, malathion and their oxon derivatives to larval Rana boylii.” “Since some of the parent pesticide compounds are already at concentrations sufficient to cause significant amphibian mortality in the Sierra Nevada, the higher toxicity of the breakdown products poses a serious problem,” said Gary Fellers, Ph.D., coauthor of the study. Donald Sparling, Ph.D., a research biologist and contaminants specialist at Southern Illinois University, and Dr. Fellers, a research biologist and amphibian specialist at the USGS Western Ecological Research Center in California, conducted laboratory tests to determine the acute toxicity – the lethal dosage causing death in 96 […]