(Beyond Pesticides, March 22, 2022) German cockroaches collected from U.S. residential homes have evolved resistance mechanisms so strong that many can consume ten times the pesticide required to kill a laboratory-susceptible strain and still not die. These are the findings of recent research published in the Journal of Economic Entomology, which focused on determining cockroach resistance levels to commonly used gel bait insecticides in infested Southern California homes. The findings underscore the importance of an integrated approach to cockroach management that recognizes and responds to pest ecology, rather than search for an ever-elusive silver bullet. Researchers collected five different strains of German cockroaches from various locations around Southern California, two from public housing and three from apartment dwellers. All sites had long-standing cockroach infestations, with varying treatment histories that generally included significant use of common gel bait insecticides. Tests were conducted on male cockroaches as they are gregarious foragers and thus more susceptible to baited food; it was indicated that if an insecticide cannot kill a male, it is highly unlikely to kill a juvenile or female roach. A separate group of cockroaches reared in the laboratory and never exposed to insecticides was used as a baseline for comparison. This […]

(Beyond Pesticides, March 10, 2022) Glyphosate and glyphosate-based herbicides (GBH) like Roundup® induce DNA damage and alter biological mechanisms (gene regulatory microRNAs [miRNAs or miRs]) associated with cancer development. According to the study published in Toxicological Sciences, DNA damage mainly occurs through oxidative stress from GBH exposure. Moreover, DNA damage and other biological mechanisms that cause carcinogenicity (cancer) occur at doses assumed “safe” by pesticide regulators such as the U.S. Environmental Protection Agency (EPA). Glyphosate is the most commonly used active ingredient worldwide, appearing in many herbicide formulas, not just Bayer’s (formerly Monsanto) Roundup®. The use of this chemical has been increasing since the inception of crops genetically modified to tolerate glyphosate over two decades ago. The toxic herbicide readily contaminates the ecosystem with residues pervasive in food and water commodities. In addition to this study, literature proves time and time again that glyphosate has an association with cancer development, as well as human, biotic, and ecosystem harm.  Study lead author Michael Antoniou, Ph.D., cautions, “Our results are the first to simultaneously show glyphosate and Roundup toxicity in a whole mammalian animal model system and provide a mechanism – oxidative stress – by which DNA damage has been observed in other systems, such as mammalian […]

(Beyond Pesticides, February 24, 2022) Widespread, intensive pesticide use for mosquito control has allowed genetic mutations to persist among mosquito populations, causing subsequent resistance to future chemical exposure. According to a study published in Scientific Reports, two common species of female mosquitoes learned to evade pesticides following non-fatal exposure through smell. More concerning is the survival rate of these pre-exposed mosquitoes, as it is more than double that of unexposed mosquitoes. Insects, including mosquitoes, use various sensory and cognitive abilities like vision, smell, and hearing to navigate the ecosystem for survival and reproduction. Mosquitoes associate sensory stimuli like smell to a positive or negative experience, thus facilitating a response. Considering the two species of mosquitoes in this study are a vector for numerous diseases in humans, including dengue and Zika and West Nile viruses. Hence, this study highlights the significance of addressing pest resistance in pest management strategies, particularly to mitigate disease exposure and effects. The study notes, “[The] findings highlight the importance of mosquito cognition as determinants of pesticide resistance in mosquito populations targeted by chemical control.” It is essential to understand insect behavior that propagates vector-borne disease transmission that exacerbates the widespread public health crisis. Scientists attribute memory […]

(Beyond Pesticides, February 18, 2022) A new analysis conducted by Safer States, and reported on by Environmental Health News (EHN), concludes that in 2022, at least 32 states will consider 210 potential laws to ban or restrict one category of so-called “legacy” chemicals — the PFAS (per- and polyfluoroalkyl substances) family of compounds. “Legacy” or “forever” chemicals are those whose historical use, including many decades ago in some instances, has led to their toxic persistence in the environment and in organisms. In recent years, scientists, health and environment advocates, and policy makers have begun to recognize these as very serious contaminants, and to call attention to their ubiquity and impacts. Beyond Pesticides has identified multiple instances of such “legacies” (including those related to the production of pesticides and particularly, the infamous DDT), and will here discuss both PFAS, and concerns about such legacy chemicals as they may impact food producers. The term “legacy” often connotes the ongoing influence or impact — generally salutary — of an individual’s activity, or a set of principles or activity inherited from one’s forebears. It is an apt description, minus the “salutary” part, for legacy chemicals — toxic “gifts that keep on giving” via persistent contamination […]

(Beyond Pesticides, January 12, 2022) Well water in agricultural regions of Sri Lanka is contaminated with highly hazardous insecticides and associated with a decline in kidney function, according to research published in npj Clean Water this month. This finding is the latest piece in an ongoing ‘puzzle’ regarding the epidemic of chronic kidney disease of unknown origins in Sri Lanka and other developing countries in agricultural regions. Although the exact etiology of the disease has not been confirmed, a number of scientific studies have pointed the finger at industrial agriculture, increasingly finding evidence of chronic pesticide exposure in affected populations.   To better understand the connection between agrichemical exposure and kidney health, researchers enrolled 293 individuals from Wilgamuwa, Sri Lanka into a prospective study. Baseline data was retrieved on occupational and environmental exposure factors, focusing in on the water source individuals used at their homes. Samples of each participant’s household wells were taken and analyzed for the presence of pesticides. Of the wells sampled, 68% were found to contain pesticides. Further, every well where pesticides were detected had at least one pesticide recorded above global drinking water guidelines. The chemicals found were also some of the most toxic pesticides to […]

(Beyond Pesticides, January 4, 2021) Chronic exposure to pesticides used in conventional forestry operations runoff and harm soft shell clams, according to a recent study published in Science of the Total Environment, entitled “The silence of the clams: Forestry registered pesticides as multiple stressors on soft-shell clams.” Rather than focusing on the impact of a single chemical, researchers analyzed the combined effects of several pesticides. “This is an important data gap to fill as research on these compounds’ toxicity typically focuses on individual compound effects at high concentrations to determine lethality, which while necessary for understanding compound toxicity, can miss sublethal effects that can have long term impacts on these systems,” said lead author Allie Tissot of Portland State University. The soft shell clam, Mya arenaria, is found to be widespread in coastal areas in both the western and eastern U.S., and is often eaten in stews or chowders. A recent study found a range of chemical contaminants detected in Oregon populations of these species, prompting researchers to further investigate the impact of these exposures. An experiment was set up with tanks to mimic a seabed, and eight different groups of 11 clams were established and treated with various amount […]

(Beyond Pesticides, December 10, 2021) In a win for pollinators, a California Superior Court has issued a ruling that sulfoxaflor, a systemic pesticide that is “field legal” but “bee lethal,” can no longer be used in the state. The suit was brought by the Pollinator Stewardship Council and the American Beekeeping Federation. The ruling of the Superior Court of the State of California for Alameda County finds that the argument of the petitioners — that sulfoxaflor approval decisions by the California Department of Pesticide Regulation (DPR) violated the California Environmental Quality Act (CEQA) — is valid. Eliminating this highly bee-toxic pesticide from use in the state is expected to protect not only native bees and other pollinators (including Monarch butterflies in early Spring), but also, the many millions of managed-colony bees that are transported to California for pollination of almond and other crops. The suit was filed against DPR, Corteva inc., Dow Agrosciences LLC, the Siskiyou County Department of Agriculture, and James E. Smith as Siskiyou County Agricultural Commissioner. Having found for the petitioners’ request for a Writ of Mandate (a court order requiring a lower court or public authority to perform its statutory duty), the court instructed the petitioners to […]

(Beyond Pesticides, December 7, 2021) One exposure. That’s all it takes for wild bees to experience declines in reproduction and population growth from neonicotinoid insecticides, according to research recently published in the Proceedings of the National Academy of Sciences (PNAS). This incredible sensitivity is exactly the sort of process that could rapidly drive pollinator species into extinction. It is the sort of finding that one would expect government agencies tasked with protecting the environment to discern. Yet, regulators at the U.S. Environmental Protection Agency’s (EPA) Office of Pesticide Programs have consistently failed to listen and meaningfully respond to the latest science. As this is done, the agency is fully aware that ever more pollinators are slated for endangered status, jeopardizing our agricultural economy, ecosystem stability, and the joy we all gain from watching our favorite pollinators flit about the landscape. Over the course of two years, researchers established a crossed experiment with ground-nesting blue orchard bees (Osmia lignaria). These pollinators, native to North America, overwinter and nest in narrow holes or tubes, making them particularly sensitive to ground-based pesticide applications. Researchers conducted their study during the first year by exposing a group of larval bees to the neonicotinoid imidacloprid through […]

(Beyond Pesticides, November 11, 2021) Evidence is building that so-called ‘inert’ ingredients in pesticide formulations are harming pollinators and undermining regulatory determinations that designate products as ‘bee-safe.’ According to a new study published in Scientific Reports, the fungicide Amistar causes lethal and sublethal effects that can be primarily attributed not to its active ingredient azoxystrobin, but to alcohol ethoxylates, a co-formulant, or inert ingredient intentionally added to a pesticide formulation. While the U.S. Environmental Protection Agency (EPA) utilizes a ‘bee advisory box’ on pesticide labels to indicate danger to pollinators, results of this and previous studies on inert ingredients underline how EPA’s ‘cute little bee icon’ is little more than window dressing for massive regulatory failures and a pollinator crisis that has shown no signs of abating. Scientists at Royal Holloway University in London, UK began their study with three packaged colonies of Bombus terrestris, a European bumblebee often bred for commercial use in greenhouses throughout the world. In order to suss out differences in toxicity between the various ingredients in the formulated Amistar fungicide, bees were separated into multiple groups. One group acted as a positive control, and was dosed with dimethoate, a pesticide known to be highly toxic […]

(Beyond Pesticides, November 10, 2021) The diversity and abundance of freshwater aquatic insects plunges when commonly used neonicotinoid (neonic) insecticides leach into waterways, finds research published in the Proceedings of the National Academy of Sciences this month. While this is the latest study exploring the effects of neonicotinoids in the field at real-world exposure levels, it is far from the first to show unacceptable hazards to wildlife and ecological health. As research on neonics piles up, advocates are watching in dismay as regulators at the Environmental Protection Agency (EPA) fail to respond to the science and allow indiscriminate poisoning to continue. To determine how neonicotinoids affect critical aquatic species near the bottom of the food chain, researchers created a series of 36 experimental ditches, split into four groups of nine. One group acted as a control and received no pesticide, and each other group received, 0.1, 1.0, or 10 parts per billion (ppb) of the thiacloprid, a neonic insecticide often cited by industry and regulators as having lower toxicity concerns than other neonicotinoids. Mimicking a pulse that may come from a nearby insecticide application, each group of ditches was dosed every two weeks for a period of three months. Scientists […]

(Beyond Pesticides, November 3, 2021) With a significant and increasing share of the U.S. population reporting sensitivities to certain chemicals, a team of researchers at University of California (Irvine), University of Texas (San Antonio), and the Massachusetts Institute of Technology is working to better understand how these symptoms develop. Although referred to by several names over the years, including Multiple Chemical Sensitivity (MCS) and Idiopathic Environmental Illness, medical professionals are now referring to the disease as Chemical Intolerance, or Toxicant Induced Loss of Tolerance (TILT), to better represent the disease process and range of nervous system symptoms that individuals develop to low level chemical exposures. “We established evidence of this previously understudied disease process,” said Shahir Masri, Sc.D, at University of California, Irvine. “Our insights will help public health scientists, physicians and policymakers better understand how to minimize harmful exposures and prevent future disease.” TILT is characterized by a two-step process. First, there is an “initiation exposure event,” whereby an individual is either repeatedly exposed to low levels of certain chemicals, or experiences a major exposure incident. In the second stage, affected individuals are “triggered” even by minute exposures, not only to the chemical that affected them in the first […]

(Beyond Pesticides, October 19, 2021) Exposure to the insecticide malathion increases risk of developing chronic kidney disease (CKD), according to a study recently published in the International Journal of Environmental Research and Public Health. According to study co-author Nicholas Osborne, PhD, CKD is on the rise in developing countries in Southeast Asia and Central America, and, “[n]early one in 10 people in high income countries show signs of CKD, which is permanent kidney damage and loss of renal function.” Although CKD risk increases with age, and is associated with other health factors like smoking, heart disease, and diabetes, cases without clear cause are increasingly common, indicating the that environmental factors are likely playing a role. Researchers began with data drawn from the United States’ National Health and Nutrition Examination Survey (NHANES), an ongoing study that assesses Americans’ health and nutritional status through interviews, physicals, and other health tests. Urine samples taken from individuals enrolled in NHANES 2001-2004 and 2007-2010 (tests within years between these dates did not analyze specific pesticides) were reviewed for the presence of pesticides, and compared against data collected on kidney function. In addition to malathion, 2,4-D, chlorpyrifos, and 3-PBA, the major metabolite for most synthetic pyrethroid insecticides, […]

(Beyond Pesticides, October 1, 2021) With this article, Beyond Pesticides rounds out its coverage of recent revelations about compromised science integrity at the U.S. Environmental Protection Agency (EPA). As Sharon Lerner reports in her September 18 (and third in a series) article in The Intercept, new documents and whistleblower interviews reveal additional means by which EPA officials have gone out of their way to avoid assessing potential health risks of hundreds of new chemicals. Ms. Lerner writes that “senior staff have made chemicals appear safer — sometimes dodging restrictions on their use — by minimizing the estimates of how much is released into the environment.” Beyond Pesticides regularly monitors and reports on scientific integrity at EPA, including two recent articles that reference Ms. Lerner’s The Intercept reporting; see “EPA Agenda Undermined by Its Embrace of Industry Influence,” and “Whistleblowers Say EPA Managers Engaged in Corrupt and Unethical Practices, Removed Findings, and Revised Conclusions.” Whistleblowers had already provided evidence of agency malfeasance, particularly in EPA’s New Chemicals Division (NCD), such as “managers and other officials . . . pressuring [EPA scientists] to assess chemicals to be less toxic than they actually are — and sometimes removing references to their harms from […]

(Beyond Pesticides, September 28, 2021) A widely used mosquito pesticide may exacerbate the effect of the Zika virus on fetal brain development, according to research published by an international team of scientists in the journal Environmental Pollution. Pyriproxyfen, an insect growth regulator often used as a mosquito larvicide, is registered for use in hundreds of commonly used pesticide products. But scientists have discovered that the pesticide’s mode of action has the potential to worsen the public health mosquito diseases the chemical aims to control. The research reinforces the extent of unknowns associated with synthetic pesticide exposure, underlining the need for a focus on nontoxic and ecological mosquito management. Scientists base their research on reports that in Brazil, during the 2015 Zika epidemic, certain areas of the country experienced higher rates of microcephaly. Microcephaly is a rare condition that causes pregnant women’s fetus to develop severe cranial deformities, alongside a range of other symptoms that include vision problems, hearing loss, feeding issues, developmental delays and seizures. The present study aimed to see how pyriproxyfen, used at higher rates in areas where microcephaly Zika cases were recorded, may interact with the virus. In an article published in The Conversation, researchers note that […]

(Beyond Pesticides, September 15, 2021) Toxic pesticide use, and glyphosate in particular, degrades the health of freshwater ecosystems by harming species that form the basis of aquatic food chains, according to research published by scientists at McGill University. In a series of studies, scientists investigated how freshwater bacteria and zooplankton were affected by varying levels the weed killer glyphosate, the neonicotinoid insecticide imidacloprid, and nutrient levels. “Because plankton form the foundation of the food chain in freshwater ecosystems, it is very important to understand how plankton communities respond to widely used pesticides,” said Jesse Shapiro, PhD, an Associate Professor in McGill’s Department of Microbiology and Immunology. “Our research shows that the structure of these communities can be impaired under currently acceptable North American water quality guidelines.” Two separate experiments were conducted under similar conditions in order to properly investigate the effects of pesticide exposure on either zooplankton or freshwater bacteria. For both studies, target species were exposed to varying rates of glyphosate, imidacloprid, or both chemicals at either high or low water nutrient levels. Researchers conducted this study by establishing a series of outdoor experimental ponds, intended to mimic freshwater ecosystems by using Lake water and evenly distributing organisms throughout […]

(Beyond Pesticides, September 9, 2021) A new study published in Toxicology Reports finds the same chemicals that disrupt the endocrine (hormone) system also disrupt the nervous system. Endocrine disruptors are xenobiotics (i.e., chemical substances like toxic pesticides foreign to an organism or ecosystem) present in nearly all organisms and ecosystems. The World Health Organization (WHO), European Union (EU), and endocrine disruptor expert (deceased) Theo Colborn, Ph.D., classify over 55 to 177 chemical compounds as endocrine disruptors, including various household products like detergents, disinfectants, plastics, and pesticides. Past research shows exposure to endocrine-disrupting pesticides adversely affects human health, from reproductive function to cancer development, and effects can span generations. However, this study is one of the few to evaluate associations between endocrine-disrupting chemicals and neurological function. Although the etiology (cause) of many sporadic (non-heritable) neurological diseases are unknown, scientists suggest exposure to environmental toxicants plays a role in disease development. Therefore, government and health officials have been urged to consider how exposure to endocrine-disrupting chemicals can impact bodily function and development apart from hormone disruption.   In the body, cells communicate through electrical or chemical signals transmitted within the nervous or endocrine system. Studies find exposure to endocrine-disrupting chemicals has a direct and indirect impact on hormone function and development. However, researchers investigated whether […]

(Beyond Pesticides, September 2, 2021) A McMaster University (Canada) study demonstrates exposure to the recently banned, commonly used insecticide chlorpyrifos promotes obesity development, even at low doses. Obesity generally occurs following a caloric imbalance between food intake, absorption, and energy expenditure. Although various factors can promote obesity, researchers suggest environmental toxicants like chlorpyrifos play a role in obesity development through protein/enzyme suppression. According to the Center for Disease Control, 42 percent of the U.S. population is obese and at risk for type two diabetes, cardiovascular (heart) disease, stroke, several cancers, and other critical health issues. Therefore, research like this highlights the significance of investigating how toxic chemical exposure can impact health to prevent adverse disease outcomes. Researchers note, “These studies suggest that the effects of environmental toxicants on the development of obesity may have been underestimated as all studies to date have been conducted in mice housed at RT [room temperature]. Future studies examining the mechanisms driving reductions in β-AR [beta adrenergic receptors] signaling and whether there are associations between BAT [brown adipose tissue] metabolic activity and CPF [chlorpyrifos] in humans will be important.” Several environmental pollutants have links to obesity development via the effects on gut health, endocrine (hormone) and metabolic system, […]

(Beyond Pesticides, September 1, 2021) A study published in Environmental Pollution finds the risk of acute childhood leukemia (AL) increases with prenatal and newborn exposure to pesticides (i.e., insecticides and herbicides). The study results support the hypothesis that chronic environmental pesticide exposure increases childhood leukemia risk up to two times. Maternal exposure has a stronger association with leukemia than childhood exposure. Insecticides and herbicides are of particular significance in increasing leukemia risk, especially for acute lymphoblastic leukemia. Although medical advancements in disease survival are more prominent nowadays, childhood AL remains the secondary cause of child mortality following physical injury. Furthermore, childhood leukemia survivors can suffer from chronic or long-term health complications that may be life-threatening. Although the etiology or cause of childhood AL involves the interaction of multiple components like lifestyle and genetics, emerging evidence indicates that environmental contaminants like pesticides (e.g., occupational exposures, air pollution, pesticides, solvents, diet, etc.) play a role in disease etiology. Pesticide contamination is widespread in all ecosystems, and chemical compounds can accumulate in human tissues resulting in chronic health effects. Children are particularly vulnerable to the impacts of pesticide exposure as their developing bodies cannot adequately combat exposure effects. Already, studies find low levels of pesticide exposure during […]

(Beyond Pesticides, August 20, 2021) The Office of the Inspector General (OIG) for the U.S. Environmental Protection Agency (EPA) has issued a damning report on the agency’s progress in protecting the population from potentially damaging endocrine disruption impacts of exposures to synthetic chemical pesticides (and other chemicals of concern). The report’s summary statement says, “Without the required testing and an effective system of internal controls, the EPA cannot make measurable progress toward complying with statutory requirements or safeguarding human health and the environment against risks from endocrine-disrupting chemicals.” This OIG report identifies and details the failings that Beyond Pesticides covered in an April 2021 Daily News Blog article, and many more — the net of which is that “we have yet to see EPA use endocrine disruption findings in pesticide registration decisions.” The OIG report chronicles a litany of failures. It finds that EPA’s Office of Chemical Safety and Pollution Prevention (OCSPP), which is responsible for testing all pesticide chemicals for endocrine disrupting activity in humans, has failed to do so. Specifically, it has not implemented a section of the Federal Food, Drug, and Cosmetic Act (FFDCA), as amended by the 1996 Food Quality Protection Act — the legislation that […]

(Beyond Pesticides, August 13, 2021) Across the pond in the UK, two years of trials with spring and winter wheat varieties have shown, according to the Farmer Scientist Network (FSN), which conducted the study as Crop Health North, that so-called “biopesticides,” alone or in combination with conventional pesticides, can be useful in generating yield and grain quality comparable to those obtained through use of conventional “crop chemistry” (aka, synthetic chemical pesticides). According to Beyond Pesticides Executive Director Jay Feldman, biopesticides are a “mixed bag,” generally poorly understood, and defined differently by various entities and stakeholders. He notes that the term can be misleading and mixes contradictory approaches, adding that, “It’s troublesome when we continue to look for product replacements or substitutions for agricultural practices that are clearly ineffective, and in the process avoid the changes necessary to transition to organic practices,” which represent the real, long-term solution to the problems efforts such as these trials seek to remedy. The project was sponsored and supported by the Yorkshire Agricultural Society (YAS) through the European Innovation Partnership (EIP-AGRI). YAS collaborated with universities, farmers, research institutes, agricultural organizations, and technology and food testing centers to conduct trials on three farms in the north of […]

(Beyond Pesticides, August 11, 2021) Multiple stressors, including pesticides, parasites, and poor nutrition, act synergistically to increase the risk of bee mortality, according to a meta-analysis recently published in the journal Nature. The findings are yet another indictment of the U.S. pesticide regulatory system’s ability to protect pollinators, as the authors note that their results, “…demonstrate that the regulatory process in its current form does not protect bees from the unwanted consequences of complex agrochemical exposure.” As scientific community continues to confirm the dangers of pesticides and other anthropogenic stressors to pollinators, it remains up to advocates and other concerned residents to get regulators and policymakers to listen to and act on these critically important conclusions.   Scientists aimed to evaluate how combinations of multiple pesticides, parasites, and lack of floral resulted in bee death or subchronic effects that impacted overall fitness (reproductive ability, colony health, etc), behavior, parasite load, or immune response. The effects of multiple stressors can be characterized as antagonistic when stressors cancel themselves out, additive when the impacts seen are what would be predicted when summing the individual effects, and synergistic when the effects are multiple times more harmful than what would be predicted additively. To […]

(Beyond Pesticides, August 4, 2021) Neonicotinoid insecticides applied to nursery plants sold at garden centers kill off wild, solitary pollinators regardless of the amount applied, according to research published in the Proceedings of the Royal Society B. The news is unlikely a surprise for those tracking the science around pollinator declines, but nonetheless a stark reminder of the lack of progress from federal regulators to stop practices that contribute to the ongoing crisis. With new science consistently showing unacceptable hazards to pollinator populations, advocates are urging Congress to take up and pass the Saving America’s Pollinators Act. Since 2006, scientists and beekeepers have singled out neonicotinoids, a class of systemic insecticides, for their role in pollinator die-off and decline. Once applied onto a seed or sprayed on a plant, neonicotinoids distribute themselves throughout the plant’s structure. This causes soft-bodied sucking insects like aphids to be killed when they eat any part of the plant. However, neonicotinoids also make their way into the pollen and nectar the plant produces, as well as the dew drops plants will secrete and pollinators will often use to grab a quick drink. The use of these insecticides on native plants sold at nursery stores throughout […]

(Beyond Pesticides, July 20, 2021) Chlorpyrifos exposure results in the expression of genetic mutations associated with autism spectrum disorder in a laboratory model, finds research published in Environmental Health Perspectives by scientists at Johns Hopkins Bloomberg School of Public Health. “This is a step forward in showing an interplay between genetics and environment and its potential role for autism spectrum disorder,” says study lead Lena Smirnova, PhD, a research associate in the Department of Environmental Health and Engineering at the Bloomberg School. The findings support reams of research already conducted that show strong associations between autism and exposure to hazardous environmental stressors like toxic pesticides. Scientists conducted their study using a ‘brain organoid’ model, which is essentially a cluster of cells artificially grown in the lab from stem cells in order to mimic a developing human brain. These tests provide certain benefits over animal testing, as they are more relevant to human disease, and can be performed faster with less cost. The organoid model also represents an improvement on typical 2d cell-based models, increasing cell survival, shelf-life, and thus providing opportunity to model for later stages of brain development. Brain organoids in this study carried a gene called CHD8, which […]