(Beyond Pesticides, October 14, 2010) Over 13,000 organizations and individuals -consumers, parents, health advocates, farmworkers and others- from across the U.S. sent a letter to the Environmental Protection Agency (EPA) yesterday calling for a ban on the insecticide chlorpyrifos and a phase out of other organophosphate (OP) pesticides. Chlorpyrifos was phased out for residential use under a 2000 agreement between EPA and Dow Agrosciences, but continues to expose farmworkers and consumers through its use in agriculture. Also on October 13, the Endocrine Disruption Exchange (TEDX), led by renowned scientist Theo Colborn, PhD, announced the addition of chlorpyrifos to its online database, Critical Windows of Development, spotlighting research that links prenatal, low dose chlorpyrifos exposure to altered health outcomes in the brain and other organs. “Human studies have now linked prenatal exposure to chlorpyrifos with mental and developmental delays emphasizing even more the urgency to remove the product from the market,” said Dr. Colborn, President of TEDX and a signatory on the letter. “Chlorpyrifos illustrates the urgent need to be cautious, prevent further exposure and protect our children from the time they are conceived onward.” Beyond Pesticides calls EPA’s 2000 chlorpyrifos settlement with Dow a classic failure of the risk assessment […]

(Beyond Pesticides, September 14, 2010) A new study confirms that indoor uses of consumer products, including pesticides, are the primary sources of indoor exposure to endocrine disruptors —chemicals that disrupt hormones and cause adverse developmental, disease, and reproductive problems— and shows that indoor levels are higher than those outdoors. Researchers from Silent Spring Institute, Columbia University, and the University of California-Berkeley measured airborne concentrations of endocrine disruptors in two California communities: Bolinas, a rural, affluent coastal town, and Richmond, a working-class city ringed by oil refineries. The study is published online in the September 1, 2010 issue of Environmental Science & Technology. The researchers analyzed 104 chemicals in 50 homes, including both chemicals that penetrate indoors from outdoor industrial and transportation sources and those from indoor use of consumer products and building materials. Similar levels of contamination were found inside homes in both communities, but outdoor levels were higher in Richmond. Among the chemicals found were pesticides, phthalates, parabens, PBDE flame retardants, and PCBs. A total of 38 pesticides are evaluated, including banned organochlorines (e.g., DDT, PCP), and current use products such as carbamates (e.g., propoxur), organophosphates (e.g., chlorpyrifos), and pyrethroids (cypermethrin). Thirteen pesticides were detected outdoors and sixteen pesticides […]

(Beyond Pesticides, August 25, 2010) Exposure to pesticides while in the womb may increase the odds that a child will have attention deficit hyperactivity disorder (ADHD), according to researchers at the University of California-Berkeley School of Public Health. Maternal metabolites of organophosphate pesticides have previously been associated with neurobehavioral deficits in children. The California researchers are studying the impact of environmental exposures on the health of women and children who live in the Salinas Valley, an agricultural region with heavy pesticide use. They tested the urine of pregnant women for pesticide residue, and then tested the behavior of their children at ages 3 ½ and 5. The 5-year-olds who had been exposed to organophosphate pesticides while in the womb have more problems with attention and behavior than did children who were not exposed. Results are published online in the study entitled, “Organophosphate Pesticide Exposure and Attention in Young Mexican-American Children,” in the journal, Environmental Health and Perspectives. Previous studies have shown that exposure to some organophosphate compounds cause hyperactivity and cognitive deficits in animals. One study published in Pediatrics earlier this year found that exposure to organophosphates in developing children might have effects on neural systems and could contribute to […]

(Beyond Pesticides, July 29, 2010) A set of mandatory rules intended to reduce pesticide use in public and private schools in Indiana is pending approval after voluntary implementation guidelines failed. The Indiana Pesticides Board submitted a draft proposal in June outlining rules to minimize pesticide exposure to students. These measures include banning the use of pesticides when students are present, keeping pesticides locked in storage areas where students do not have access, providing advance notice of pesticide applications, and using pesticides with the lowest hazards to children. Although Beyond Pesticides recommend the additional step of developing a defined Integrated Pest Management (IPM) program, these tactics represent a good first step towards pesticide reduction in schools. The Indiana School Board Association developed a set of voluntary pesticide guidelines in 2001, but while rates of adoption increased, the Indiana state chemist’s office found that some schools were not implementing those policies, or had not adopted pesticide guidelines. It is important that schools adopt a comprehensive pesticide policy because children are especially vulnerable to the health hazards associated with pesticide exposure due to their small size, greater intake of air and food relative to body weight, and developing organ systems. Several pesticides, including […]

(Beyond Pesticides, June 16, 2010) Men with certain genetic variations who were exposed to some toxic pesticides that are now largely banned run an increased risk of developing Parkinson’s disease, French scientists said Monday. In a study published in Archives of Neurology, entitled “Interaction Between ABCB1 and Professional Exposure to Organochlorine Insecticides in Parkinson Disease,” French researchers found that among men exposed to pesticides such as DDT, carriers of the gene variants are three and a half times more likely to develop Parkinson’s than those with the more common version of the gene. The scientists think the brains of people with the gene variant fail to flush out toxic chemicals as efficiently as those with common versions of the gene, suggesting that environmental as well as genetic factors are important in the risk of Parkinson’s. Alexis Elbaz, MD, PhD and Fabien Dutheil, PhD, of France’s National Institute for Health and Medical Research (INSERM) studied 101 men with Parkinson’s and 234 without the disease to look at links between organochlorine exposure and Parkinson’s disease. The study includes only men, and all of them had high levels of exposure to pesticides through their work as farmers. The scientists found the link was […]

(Beyond Pesticides, May 28, 2010) The repeated exposure to organophosphate and organochlorine insecticides can increase the risk of developing Alzheimer’s Disease (AD) or dementia later in life according to a new study published in the May issue of Neurology. The observational study entitled “Occupational exposure to pesticides increases the risk of incident AD” is one of very few studies to examine a link between pesticides and AD. Researchers lead by Kathleen M. Hayden, PhD of Duke University Medical Center examined residents 65 years and older from an agricultural community in Cache County Utah. Participants were assessed for cognitive ability at the inception of the study and again after 3, 7, and 10 years. Data showed that those repeatedly exposed to any pesticides were more likely to develop AD or dementia. Researchers found a higher incidence of AD among those exposed to organophosphates and organochlorines. The risk of AD associated with organophosphate exposure was slightly higher than the risk associated with organochlorines. Researchers also found an increase in dementia among those exposed to organophosphates or organochlorines; however this increase was not statistically significant. Dr. Hayden said that more research was necessary to determine a causal link. Organophosphates are known to reduce […]

(Beyond Pesticides, May 18, 2010) A team of scientists from the University of Montreal and Harvard University have discovered that exposure to organophosphate pesticides is associated with increased risk of Attention-Deficit Hyperactivity Disorder (ADHD) in children. Using data from the National Health and Nutrition Examination Survey, the study focused on 1,139 children from the general U.S. population and measured pesticide breakdown product levels in their urine. The authors conclude that exposure to organophosphate (OP) pesticides, at levels common among U.S. children, may contribute to a diagnosis of ADHD. “Previous studies have shown that exposure to some organophosphate compounds cause hyperactivity and cognitive deficits in animals,” says lead author Maryse F. Bouchard, a professor at the University of Montreal Department of Environmental and Occupational Health and scientist at the Sainte-Justine Hospital Research Center. “Our study found that exposure to organophosphates in developing children might have effects on neural systems and could contribute to ADHD behaviors, such as inattention, hyperactivity, and impulsivity.” Marc Weisskopf, PhD, ScD, another study author told Reuters, “What this paper specifically highlights is that this may be true even at low concentrations.” For children with a 10-fold increase in the concentration of the most common dialkyl phosphate metabolites […]

(Beyond Pesticides, March 29, 2010) Prenatal exposure to pesticides at levels that do not cause adverse health effects in the mother can lead to delayed brain developmental in the child, according to the new study, “Neurobehavioral Deficits and Increased Blood Pressure in School-Age Children Prenatally Exposed to Pesticides,” published last month in the early online edition of the journal Environmental Health Perspectives. Many pesticides are suspected of being capable of damaging the nervous system, in particular the development of a child’s brain during pregnancy and the early postnatal years of life. Now an international research team led by Philippe Grandjean, M.D., from the University of Southern Denmark and Harvard School of Public Health, has shown a delayed brain development up to two years in school children, whose mothers worked in a greenhouse during pregnancy. The exposed children also had increased blood pressure, while the mothers do not suffered adverse symptoms themselves. “The results support the notion that, in this cohort of children, prenatal exposures to pesticides are more harmful than current exposures, thereby confirming previous results of other environmental studies of neurodevelopmental toxicity and the theory of window of vulnerability of central nervous system during uterine life,” write the authors. […]

(Beyond Pesticides, March 10, 2010) A study investigating the sublethal effects of pyrethroids, bifenthrin and deltamethrin on honeybees finds that the chemicals significantly impair the pollinators’ reproduction. The researchers also point out that the concentration of each pesticide that produced adverse effects in the experiments was at or below those that bees could encounter while pollinating treated crop fields. “Effects of sublethal concentrations of bifenthrin and deltamethrin on fecundity, growth, and development of the honeybee Apis mellifera ligustica” published in the March issue of Environmental Toxicology & Chemistry, investigated the effects of the two pesticides at sublethal concentrations on fecundity, growth, and development of honeybees were examined with the feeding method for a three-year period (2006-2008). It was shown that both bifenthrin and deltamethrin significantly reduced bee fecundity, decreased the rate at which bees develop to adulthood, and increased their immature periods. Queens in the control group in 2006 laid a little more than 1,200 eggs each day, compared to not quite 900 a day in the bifenthrin group and roughly 600 per day in the deltamethrin group. In general, the hatch rate of pyrethroid-exposed eggs was also significantly depressed. The success rate of hatchlings, that is the share that […]

(Beyond Pesticides, February 18, 2010) Hundreds of dead and dying lobsters just north of the Gulf of Maine were found to have been exposed to cypermethrin, a highly toxic synthetic pyrethroid pesticide registered for agricultural and residential use that some officials think may have been illegally used in fish farming. However, the chemical, which is primarily used for indoor insect control and termites, is extremely toxic to fish and aquatic organisms and part of a family of pesticides (synthetic pyrethroids) that is increasingly showing up in water bodies at toxic levels, a cause for concern according to scientists. Area fisherman are angry and concerned, however investigators are not yet certain just how this pesticide wound up in the Bay of Fundy, which is located between the Canadian provinces of Nova Scotia and New Brunswick. The first dead lobsters were discovered last fall in Grand Manan’s Seal Cove, and only a few days later fisherman found dead lobsters in two different locations in the Bay, including about 816 kilograms of dying or dead lobsters in Deer Island’s Fairhaven Harbour. This prompted an investigation by Environment Canada that began on December 22, 2009. The department looked at samples of crab, kelp, mussels […]

(Beyond Pesticides, February 16, 2010) Pyrethroids, among the most widely-used home pesticides, are winding up in California rivers at levels toxic to some stream-dwellers, possibly endangering the food supply of fish and other aquatic animals, according to a study by researchers at the University of California, Berkeley, and Southern Illinois University (SIU). The study, “Urban and Agricultural Sources of Pyrethroid Insecticides to the Sacramento-San Joaquin Delta of California,” in the journal Environmental Science & Technology, is the first published work to document toxic levels in the water column as well as in the sediments at the bottom of streams. Pyrethroid insecticides, commonly used to kill ants and other insects around the home, have been found in street runoff and in the outflow from sewage treatment plants in the Sacramento, California area. The insecticide ended up in two urban creeks, the San Joaquin River and a 20-mile stretch of the American River, traditionally considered to be one of the cleanest rivers in the region. Although the pyrethroid levels were low, around 10-20 parts per trillion, they were high enough to kill a test organism similar to a small shrimp that is used to assess water safety. “These indicator organisms are ‘lab […]

(Beyond Pesticides, January, 21, 2009) A new study concludes that exposures during pregnancy and childhood to insecticides that target the nervous system, such as organophosphates (OPs) and carbamates, are associated with childhood brain tumors. The researchers hypothesize that this susceptibility might be increased in children with genetic variations that affect the metabolism of these chemicals. The study, “Childhood Brain Tumors, Residential Insecticide Exposure, and Pesticide Metabolism Genes,” examines whether childhood brain tumors (CBT) are associated with the functional genetic variations. The study provides evidence that exposure to insecticides, paired with specific metabolism gene variants, may increase the risk of CBT. DNA was extracted from archival screening samples for 201 cases ≤ 10 years of age and born in California or Washington State between 1978 and 1990. Insecticide exposures during pregnancy and childhood were classified based on interviews with participants’ mothers. The children’s mothers reported whether they or anyone else had chemically treated the child’s home for insects including termites, fleas, ants, cockroaches, silverfish, or “other” pests. The results are consistent with the possibility that children with a reduced ability to metabolize organophosphate and carbamate insecticides might be at increased risk of CBT when sufficiently exposed. The researchers observed multiplicative interactions […]

(Beyond Pesticides, January 11, 2010) Yet another study has been published that further supports the causative link between pesticide exposure and Parkinson’s disease. The study, funded by the National Institute of Environmental Health Sciences (NIEHS), is published in the January issue of Epidemiology. The University of California, Los Angeles researchers looked at the association between Parkinson’s disease, organophosphate pesticides and the common gene variant, paraoxonase-1 gene Leu-Met 55 polymorphism (PON1-55 MM). The findings show that study participants with two copies of gene variant have a significantly increased risk of developing Parkinson’s disease when exposed to certain organophosphate pesticides used in agriculture. The population-based case-control study examined the DNA of 351 incident cases and 363 controls from three rural counties in California. The researchers then used pesticide usage reports and a geographic information system (GIS) approach to determine the study participants’ residential exposure to organophosphates. The PON1 gene codes for an enzyme that metabolizes organophosphate pesticides. Individuals with the variant MM PONI1-55 genotype that are exposed to organophosphates exhibit more than twice the risk of Parkinson’s disease compared to carriers of wildtype or heterozygous genotype and no exposure. In regards to exposure to diazinon, carriers of variant MM PONI-55 genotype show […]

(Beyond Pesticides, December 1, 2009) A new study published in the November 2009 issue of Occupational and Environmental Medicine, adds rhinitis, the inflammation of the mucous lining of the nose, to the long list of ailments linked to pesticide exposure. “Rhinitis associated with pesticide exposure among commercial pesticide applicators in the Agricultural Health Study,” examined data from 2,245 Iowa commercial pesticide applicators and evaluated the association between rhinitis and 34 pesticides used in the past year. Seventy-four percent of commercial pesticide applicators in the study reported at least one episode of rhinitis in the past year (current rhinitis), compared with about 20-30% of the general population. Pesticide exposure and rhinitis were assessed at enrollment using two self-administered questionnaires. The first, completed at enrollment, obtained detailed information on use of pesticides on the market at the time of enrolment as well as smoking history, current agricultural activity and demographics. The second questionnaire, sent one month later, more detailed information on the pesticides, as well as medical history, including rhinitis, conjunctivitis, sinusitis and asthma. Respondents reported using 16 herbicides, 11 insecticides, five fungicides and two fumigants in the past year. Five of the pesticides were significantly positively associated with current rhinitis: the […]

(Beyond Pesticides, November 17, 2009) According to a new study by researchers at the University of Wisconsin-Madison, exposure to low levels of the organophosphate insecticide chorpyrifos during pregnancy can impair learning, change brain function and alter thyroid levels of offspring into adulthood for tested mice, especially females. The study, “Long-term sex selective hormonal and behavior alterations in mice exposed to low doses of chlorpyrifos in utero,” was led by Beyond Pesticides board member and professor of zoology and environmental toxicology, Warren Porter, PhD. Read the full analysis of the study on the Rodale Institute website. On June 8, 2000, the Environmental Protection Agency (EPA) and Dow AgroSciences, reached an agreement to stop the sale of most home, lawn and garden uses for chlorpyrifos because of its health risks to children. However, its use continues in agriculture. According to advocates, this new study provides further evidence for the need to ban chlorpyrifos and fully protect farmworkers, their families, and rural communities from the toxic hazards of this outdated, unnecessary pesticide. According to the Rodale Institute, which provided part of the funding for the study, “The new animal study accentuates the risk of ultra-low levels of the common pesticide chlorpyrifos to cause […]

(Beyond Pesticides, October 26, 2009) A new study conducted in China finds that people with organophosphate pesticides in their homes are more likely to have suicidal thoughts. According to the study, “Pesticide exposure and suicidal ideation in rural communities in Zhejiang province, China,” published in the October issue of the WHO Bulletin, there is biological evidence that chronic low-grade exposure to organophosphate pesticides, which are very easily absorbed into the body through the skin and lungs, may have adverse effects on mental health. The study was carried out in the central/coastal region of China, a relatively wealthy area with a rapidly developing economy. In a very large survey of mental health in rural community residents, participants were also asked about how they stored pesticides. The study found that people who stored pesticides at home, i.e. those with more exposure, were more likely to report recent suicidal thoughts. Supporting this, the survey also found suicidal thoughts to be associated with how easily accessible these pesticides were in the home and that the geographic areas with highest home storage of pesticides also had highest levels of suicidal thoughts in their populations. “Organophosphate pesticides are widely used around the world. They are particularly […]

(Beyond Pesticides, October 16, 2009) On October 14th, Earthjustice and Farmworker Justice filed a petition asking the U.S. Environmental Protection Agency (EPA) to set safety standards protecting children who grow up near farms from the harmful effects of pesticide drift. The groups are also asking the agency to adopt an immediate no-spray buffer zone around homes, schools, parks and daycare centers for the most dangerous and drift-prone pesticides, organophosphates. The petition was filed by the public interest law firms on behalf of farmworker groups: United Farm Workers, Oregon-based Pineros y Campesinos Unidos del Noreste, California Rural Legal Assistance Foundation, and the Farm Labor Organizing Committee, AFL-CIO as well as Physicians for social Responsibility, Washington-based Sea Mar Community Health Center, Pesticide Action Network North America, and MomsRising.org. Specifically, the petition states that the EPA has failed to address the facts that children are particularly vulnerable to pesticides according findings by the National Academy of Sciences (NAS) in 1993. Congress took recommendations from NAS and passed the Food Quality Protection Act in1996, requiring EPA to “ensure that there is a reasonable certainty that no harm will result to infants and children from aggregate exposure” to pesticides. However, while EPA has made some […]

(Beyond Pesticides, September 29, 2009) A new study reveals that children exposed to agricultural pesticides applied near their home have up to twice the risk of developing the most common form of childhood leukemia, according to the Northern California Cancer Center (NCCC). The study, “Residential proximity to agricultural pesticide applications and childhood acute lymphoblastic leukemia,” published in the October issue of Environmental Research, used a unique California database to reveal an elevated risk in acute lymphoblastic leukemia (ALL) among children living near applications of certain categories of pesticides used in agriculture. The study, led by Rudolph Rull, Ph.D., shows an elevated risk of ALL associated with moderate exposure, but not high exposure, to pesticides classified as organophosphates (odds ratio (OR) 1.6), chlorophenoxy herbicides (OR 2.0), and triazines (OR 1.9), and with agricultural pesticides used as insecticides (OR 1.5) or fumigants (OR 1.7). California is one of the few states in the country that requires active reporting of pesticide applications, including time, place, and the type and amount of pesticide used. For this study, researchers were able to link children’s entire residential histories from birth to the time of case diagnosis to this pesticide-use reporting database and identify agricultural pesticides that […]

(Beyond Pesticides, September, 15, 2009) On September 11, 2009, the Environmental Protection Agency (EPA) announced plans to place additional limitations on the use of three organophosphate pesticides ”” chlorpyrifos, diazinon and malathion ”” to protect endangered and threatened salmon and steelhead in California, Idaho, Oregon and Washington. The announcement comes in response to a series of lawsuits brought by Pacific Coast Federation of Fishermen’s Associations (PCFFA), the Northwest Coalition for Alternatives to Pesticides, and other salmon advocates, with legal representation from Earthjustice, aimed at removing toxic pesticides from salmon spawning streams throughout the northwest. In response to the litigation, the National Marine Fisheries Service (NMFS) in November of 2008 released a “biological opinion” that set forth a plan for protecting Pacific salmon and steelhead from three toxic organophosphate pesticides. That decision came after almost a decade of legal wrangling between salmon advocates led by Earthjustice and the federal government. The biological opinion prescribed measures necessary to keep these pesticides out of water and to protect salmon populations in Washington, Oregon, California, and Idaho. The announcement from EPA moves this work forward. Although the experts at NMFS recommended prohibiting aerial applications of the three pesticides within 1,000 feet of salmon waters […]

(Beyond Pesticides, July 29, 2009) A new study by researchers at the Lombardi Comprehensive Cancer Center at Georgetown University finds a higher level of common household pesticides in the urine of children with acute lymphoblastic leukemia (ALL), a cancer that develops most commonly between three and seven years of age. The findings are published in the August issue of the journal Therapeutic Drug Monitoring. Researchers, in the study entitled, “Pediatric Acute Lymphoblastic Leukemia and Exposure to Pesticides,” caution that these findings, which do not establish a cause-and-effect relationship, suggest an association between pesticide exposure and development of childhood ALL. “In our study, we compared urine samples from children with ALL and their mothers with healthy children and their moms. We found elevated levels of common household pesticides more often in the mother-child pairs affected by cancer,” says the study’s lead investigator, Offie Soldin, Ph.D., an epidemiologist at Lombardi. Dr. Soldin cautions, “We shouldn’t assume that pesticides caused these cancers, but our findings certainly support the need for more robust research in this area.” Previous studies have found that exposures to certain pesticides increases the risk of developing certain cancers and degenerative diseases. The study was conducted between January 2005 and […]

(Beyond Pesticides, July 17, 2009) High levels of pyrethroid pesticides in the Sacramento-San Joaquin River Delta, the number one river system on America’s Most Endangered Rivers List of 2009, has been linked to heavy urbanization in the region. Leading a study to understand the collapse of the delta’s ecosystem, University of California-Berkeley toxicologist Donald Weston, Ph.D. found that these pesticides most likely reached the river from urban storm drains, collecting household pesticide disposal and runoff from lawns of 1.4 million residents in the Sacramento region. Five years ago, a study by Dr. Weston and his colleague Michael J. Lydy, Ph.D of Southern Illinois University in Carbondale found that synthetic phyrethroids were collecting in river and creek sediments at levels that are toxic to bottom dwelling fish. Current research holds that there are enough pyrethroids to kill tiny shrimp, which are said to be the first link in the aquatic food chain. Pyrethroids are synthetic versions of pyrethrin, a natural insecticide found in certain species of chrysanthemum. It initially came on the market as a ”˜safer’ alternative to the heavily regulated and highly toxic organophosphates, such as diazinon and chlorypyrifos. Despite the fact that there are plenty of effective pest control […]

(Beyond Pesticides, June 25, 2009) Although it is known that infants are more susceptible than adults to the toxic effects of pesticides, this increased vulnerability may extend much longer into childhood than expected, according to a new study by researchers at the University of California, Berkeley. Among newborns, levels of paraoxonase 1 (PON1), an enzyme critical to the detoxification of organophosphate pesticides, average one-third or less than those of the babies’ mothers. It was thought that PON1 enzyme activity in children approached adult levels by age two, but instead, the UC Berkeley researchers found that the enzyme level remained low in some individuals through age seven. Based upon the findings, reported in the journal Environmental Health Perspectives, the study authors recommend that the U.S. Environmental Protection Agency (EPA) re-evaluate the current standards for acceptable levels of pesticide exposure. “Current EPA standards of exposure for some pesticides assume children are three to five times more susceptible than adults, and for other pesticides the standards assume no difference,” said Nina Holland, Ph.D., UC Berkeley adjunct professor of environmental health sciences and senior author of the paper. “Our study is the first to show quantitatively that young children may be more susceptible to […]

(Beyond Pesticides, June 8, 2009) A new epidemiological study finds that Parkinson’s disease patients who have been exposed to pesticides through their work show elevated rates of the disease. The researchers find that French farmworkers have nearly double the risk for the disease if exposed to pesticides, with a dose-effect for the number of years of exposure. When they looked at the three major classes of pesticides (insecticides, fungicides and herbicides), they find that the farmworkers who used insecticides had over a two-fold increase in the risk of Parkinson’s disease. A slightly higher risk is found for exposure to organochlorine insecticides. According to the study, these associations are stronger in men with older onset Parkinson’s disease than in those with younger onset Parkinson’s. The study, “Professional Exposure to Pesticides and Parkinson’s Disease,” published in Annals of Neurology, involved individuals affiliated with the French health insurance organization for agricultural workers who were frequently exposed to pesticides in the course of their work. Occupational health physicians constructed a detailed lifetime exposure history to pesticides by interviewing participants, visiting farms, and collecting a large amount of data on pesticide exposure. These included farm size, type of crops, animal breeding, which pesticides were used, […]